摘要
实验旨在观察预热休克对一次性大负荷运动造成大鼠骨骼肌运动性损伤是否具有保护作用.实验采用大鼠热休克模型,用物理加热方法使大鼠体温升高到40.5℃～41.5℃,持续10～15 min,在室温下恢复24 h后进行一次性大负荷运动(速度为27 m/min,坡度0°,时间为90min).通过对比预热和室温组大鼠运动后即刻、24 h、48 h血清中的肌酸激酶(CK)、乳酸脱氢酶(LDH),来确定预热休克对大鼠运动所致骨骼肌损伤是否具有保护作用,以及是否能够加速骨骼肌运动性损伤的恢复.结果发现,预热组运动后即刻、24 h,血清中激酶和乳酸脱氢酶活性均比室温组低(P＜0.05);运动后48 h,预热组较室温组血清中肌酸激酶和乳酸脱氢酶活性略低,没有显著性差异,两组肌酸激酶值比室温基础组略高,也没有显著性差异;预热运动后48 h组乳酸脱氢酶与室温基础组相比略高,没有显著性差异;室温运动后48 h组乳酸脱氢酶较室温基础组明显偏高,具有显著性差异(P＜0.05).提示,预热休克对于一次性大负荷运动所致的大鼠骨骼肌运动性损伤具有保护作用,并且能够促进骨骼肌运动性损伤的恢复.
The main purpose was to investigate whether heat shock had protection on exercise injury of skeletal muscle for 1 maximal training load in rat. All rats of heat groups were subjected to whole-body hyperthermia at 40. 5℃-41. 5℃ for 10-15 minutes. Rats were allowed to recovery from whole-body hyperthermia for 24 hours. Skeletal muscle was obtained from rats at right now,24 hours and 48 hours after heat shock to verify expression of HSP70. The result showed that comparing with the heating group ,exercise protocols of plasma enzymes resulted in significantly increase immediately and 24 hours after running,while 48 hours after exercise ,all protocols returned to pre-exercise level except for that activity of LDH. The activity of plasma CK and LDH in heating group were significantly decreased comparing with normal temperature group. However, the activity of plasma CK and LDH has no significant difference between two groups 48 hours after exercise. It suggested that heat shock had protection on exercise injury of skeletal muscle for 1 maximal training load in rat and improved the recovery of exercise injury of muscle.
出处
《体育科学》
CSSCI
北大核心
2005年第9期53-55,82,共4页
China Sport Science
基金
南京大学生物医药技术国家重点实验室开放项目。
关键词
热休克
骨骼肌
损伤
保护
鼠
动物实验
heat shock
skeletal muscle
injury
protection
rat
animal experiment
