摘要
动态观察电针穴位对创伤大鼠神经内分泌免疫功能的影响。用手术创伤大鼠的模型，电针刺激＂足三里＂、＂阑尾穴＂，经３Ｈ－胸腺嘧啶核苷孵育分析，测定巨噬细胞分泌白介素－１（ＩＬ─１）的功能及外围血β─内啡呔和皮质酮水平。结果：创伤组２、４、８、１２ｈ大鼠胸腔巨噬细胞（ＭΦ）分泌ＩＬ─１的功能明显增强（Ｐ＜０．０５，Ｐ＜０．００１）。各时期的血浆β─内啡肽（β─Ｅｎｄ）和皮质酮（ｃｓ）含量极明显升高（Ｐ＜０．０５；Ｐ＜０．００１），创伤大鼠经电针后，１、２、４ｈ，ＩＬ─１显著升高（Ｐ＜０．０５），８ｈ与正常大鼠巨噬细胞分泌ＩＬ─１的功能无区别，较创伤组大鼠提早４ｈ恢复正常功能，β─Ｅｎｄ含量各时相均明显低于创伤组大鼠水平（Ｐ＜０．０５；Ｐ＜０．００１）。皮质酮的含量１、２、４ｈ明显低于创伤组大鼠水平（Ｐ＜０．０５；Ｐ＜０．００１）。结论：电针穴位对手术创伤引起的机体应激状态具有良性调节作用。
PURPOSE To study the effect of electroacupuncture(EA) on immuneneuroendocrinal function after surgical trauma in rats.METHODS The 3H-TdR incubated assay was applied to measure the LPS-induced releasing of IL-1 from peritoneal macrophage. Using the radiooimmunoassay to determine the level of plasma corticosterone(Cs) and β─Endorphin (End).RESULTS The results showed that the IL─1 release from peritoneal macrophage was increasing at 2, 4, 8, 12 hours after surgical trauma (P<0.05, P<0.001). At 1, 2, 4, 8, 12 hours,the plasma β─End and Cs were increased as compared with control group (P<0.05,P<0.001).Using EA of Zusan li and Lanwei points(2Hz) for 30 min, it was found that the IL─1 level was only increased at 1, 2, 4 hours after surgical travma, but at 8 hours the IL─1 level was recovered to normal. Compared with the data from the same time (1,2,4,8,12 hours) in the trauma group, the increment of concentration of β─End and Cs is lower in EA group.CONCLUSIONS The results suggest that EA could exert regulatory effect towards normal during stress caused by surgical trauma.
出处
《上海医科大学学报》
CSCD
1996年第1期27-30,共4页
Journal of Fudan University(Medical Science)
基金
攀登计划项目资助
关键词
电针
创伤
细胞介素
Β内啡肽
electroacupuncture
surgical trauma
IL-1
β-Endorphin
corticosterone
