摘要
[目的]探讨性成熟前期褪黑素(melatonin,MT)分泌不足所诱导的神经、内分泌变化与肾阴虚证的关系。[方法] 将30只离乳后的SD大鼠随机分为正常组、模型组、滋肾阴清相火方药组(中药组);将模型组和中药组大鼠24 h/d连续光照30 d,中药组大鼠同时给予滋肾阴清相火方药(13.3 g·kg-1·d-1)灌胃,30 d后于夜间(0:00-2:00)取血,观察大鼠血液中MT、睾酮(testosterone,T)、肾上腺素(epinephrine,E)和去甲肾上腺素(norepinephrine,NE)含量的变化。[结果] 模型组大鼠体质量和血液中MT浓度下降,T、NE、E含量升高,与正常组比较均有显著性差异(P<0.05或P<0.01);给予滋肾阴清相火方药可升高大鼠体质量和血中MT浓度,降低T、NE、E含量,与模型组比较均具有显著性差异(P<0.05 或P<0.01)。[结论]连续光照可减少性成熟前期大鼠血液中MT浓度,同时升高T、NE和E含量,而滋肾阴清相火的中药可使其部分恢复,反证了性成熟前期持续光照导致的MT等分泌不足的改变类似于中医肾阴虚相火旺的证候。
[ Objective] To explore the relationship between neuroendocrine changes caused by melatonin （MT） hytpo-secretion and kidney-yin deficiency syndrome during prepubescence. [ Methods ] Thirty SD rats after ablactation were randomly divided into normal group （group A）, model group （group B） and herbal medicine group （group C, treated with kidney-yin-nourishing and deficiency-fire-clearing herbs）. Rats in groups B and C were exposed to light continuously for 30 days and simultaneously group C was given kidney-yin-nourishing and deficiency-fire-clearing herbal medicine （13.3 g·kg^-1·d^- 1, qd） for 30 days . After treatment, blood samples were collected at 0 ： 00-2 ： 00 o＇clock to observe the changes of blood MT, testosterone （T）, epinephrine （E） and norepinephrine （NE） levels. [Results] Body weight and blood MT level decreased, and T, NE and E levels increased in group A （P 〈 0.05 or P 〈 0.01 compared with group B）. Kidney-fin-nourishing and deficiency-fire-clearing herbal medicine increased body weight and blood MT level, and decreased T, NE and E levels （P 〈0.05 or P 〈0.01 compared with group B）. [Conciusion] Continuous exposure to light decreases blood MT level, and increases T, NE and E levels in prepubeseent rats; kidney-yin-nourishing and deftciency-fire-clearing herbal medicine can counteract the above changes. This indicates that manifestations in rats with MT hypo-secretion induced by light exposure during prepubescence are similar to the syndrome of kidney-yin deficiency and deficiency-fire hyperactivity.
出处
《广州中医药大学学报》
CAS
2005年第5期410-412,共3页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
国家中医药管理局资助项目(编号:02-03JP35)
广州中医药大学总体规划重点项目(编号:GH0202)
关键词
肾阴虚/诊断
褪黑激素/分泌
疾病模型
动物
大鼠
KIDNEY-YIN DEFICIENCY/diagnosis
MELATONIN/secmtion
DISEASE MODELS, ANIMAL
RATS
