摘要
目的:研究碱基切除修复基因XRCC1多态性与肺癌易感性的关系。方法:采用病例-对照研究,收集太原市原发性肺癌患者111例为病例组,同时随机抽取210名健康居民作为对照组,并进行流行病学调查。应用PCR-RFLP方法分析由内切酶MspI识别XRCC1基因Arg399Gln位点的多态性,比较不同基因型与肺癌易感性的关系,以及基因多态性与吸烟之间对肺癌易感性的交互作用。结果:XRCC1密码子399杂合基因型Arg/Gln可能对鳞癌有较弱的保护效应,并可能降低吸烟者患肺癌的危险性。而纯合突变基因型Gln/Gln与和吸烟的存在协同作用可显著提高肺癌的危险度。结论:碱基切除修复基因XRCC1密码子399的多态性可能会对肺癌易感性产生影响,并可能与吸烟量之间存在一定的协同作用。
Purpose： The current study analyzed the polymorphisms of DNA repair gene XRCC1 and relationship hetween genetic variations and susceptibility to lung eancer Methods：A ease-control study of 111 patients with lung cancer and 210 normal residents as controls was conducted to deteet XRCC1 polymorphisms at Arg399Gln loci.Genotypes were analyzed by PCR-based restrietion fragment length polymorphism（RLFP）techniques.Results：XRCC1 codon 399 heterozygous genotype Arg/Gln might have weaker protection effect on squamous cell careinoma and reduce the risk of lung eancer on smokers.Homozygous genotype Gln/Gln of XRCC1 codon 399 might increase the risk of lung cancer and have synergismight contribute to the susceptibility to lung cancer and have synergitie with smoking.Conclusion：The results demonstrated that genetic polymorphism of XRCC1 DNA repair gene might contrilbute to the susceptibility to lung cancer and have synergistic effect with somking.
出处
《中国癌症杂志》
CAS
CSCD
2005年第4期335-338,共4页
China Oncology
关键词
XRCC1基因
基因多态性
肺癌易感性
XRCC1 gene
Genetic polymorphism
susceptibility to lung cancer
