摘要
目的探讨Th1/Th2细胞因子在日本血吸虫可溶性虫卵抗原(SEA)致敏树突状细胞(DC)免疫小鼠抗感染中的作用。方法采用SEA致敏DC免疫BALB/c小鼠,ELISA分别检测免疫前、后小鼠血清干扰素(IFN)-γ和白细胞介素-4(IL-4)的含量,脾细胞培养法检测经刀豆蛋白A(ConA)和SEA刺激后,小鼠脾细胞分泌IFN-γ和IL-4的水平。结果SEA致敏DC免疫后血清IFN-γ的水平无明显变化,而IL-4的含量明显增加(P<0.01);经ConA、SEA刺激,SEA致敏DC免疫组脾细胞产生较高水平的IL-4(P<0.01),而IFN-γ水平与其他组无明显变化。结论SEA致敏DC免疫主要诱导Th2免疫反应,在抗血吸虫感染保护性免疫中起主要作用。
Objective To explore the effect of Th1/Th2 cytokines in the protective immunity against infection of Schistosoma japonicum in mice immunized with soluble eeg antigen（SEA）pulsed dendritic cells（D C）. Methods BALB/c mice were immunized with SEA pulsed DC. ELISA kits were used to examine the levels of IFN-γ and IL-4 before and 4 weeks after immunization. By the culture of spleen cells, IFN-γ and IL-4 after stimulation with SEA were quantified by ELISA kits. Results The level of IL-4 was increased significantly after immunized with SEA pulsed DC（ P〈0.01 ）. There was not obvious change in the level of IFN-γ. With spleen cells from SEA pulsed DC vaccinated mice, high level of IL-4 was produced in response to ConA and SEA（ P〈0.01）, while there was no difference between group of SEA pulsed DC immunization and control group. Conclusion SEA pulsed DC could induce Th 2 immune response which might play an important role in the protective immunity against infection of Schistosoma japonicum.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2005年第9期1066-1067,共2页
Chinese Journal of Public Health
基金
湖北省教育厅2004年重点课题资助(2004D004)
