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呋喃二氢吡啶二羧酸酯对血管紧张素Ⅱ诱导血管平滑肌细胞增殖的抑制作用

Inhibitory effects of diethyl-2,6-diethyl-4-furny-l, 4-dihydropyridine-3, 5- dicarboxylate on proliferation of cultured vascular smooth muscle cells induced by angiotension II
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摘要 目的:观察呋喃二氢吡啶二羧酸酯(EFDP)对血管紧张素II(AngⅡ)诱导血管平滑肌细胞(VSMC)增殖的影响。方法:采用培养的兔VSMC,应用3H-TdR掺入法,观察在AngII促进VSMC增殖过程中,EFDP对VSMC DNA合成的影响及其时间效应。结果:AngII可促进处于静止状态的兔VSMC的DNA合成,36 h时细胞DNA的合成达到高峰。EFDP对AngII诱导的VSMC增殖有显著的抑制作用,并呈现出明显的浓度依赖关系,随着EFDP浓度的升高,VSMC增殖的抑制率也逐渐增加,36 h时,78.40μmol/L的EFDP对VSMC增殖的抑制率为21.37%。结论:EFDP可抑制AngII诱导兔VSMC的增殖,并存在一定的量效依赖关系及时间反应性。 AIM： To investigate effects of diethyl-2,6-diethyl-4-furny-1, 4-dihydropyridine-3, 5- dicarboxylate （ EFDP ） on proliferation of cultured vascular smooth muscle cells （ VSMCs ） stimulated by angiotension Ⅱ（ AngⅡ ）. METHODS ： The rabbit VSMCs were cultured and the inhibitory effect of EFDP on DNA synthesis in vascular smooth muscle cells stimulated by Ang Ⅱ were assayed at different times with different concentrations of EFDP by using ^3H-TdR incorporation in vitro. RESULTS： Ang Ⅱ could significantly promote DNA synthesis in quiescent rabbit VSMCs, with a maximal response at 36th h. EFDP significantly inhibited DNA synthesis of VSMCs in a dose-dependent manner, with a maximal response at a concentration of 78.40μmol/L and at 36th h when the inhibitory rate was 21.37%. CONCLUSION ： EFDP significantly inhibits DNA synthesis of ribbit VSMCs induced by AngⅡ in a dose and time dependent manner.
作者 张殿新 李兰荪 贾国良 任雨笙 刘兵
机构地区 第四军医大学西京医院心内科 第二军医大学长征医院心内科
出处 《心脏杂志》 CAS 2005年第4期341-344,共4页 Chinese Heart Journal
关键词 呋喃二氢吡啶二羧酸酯 血管平滑肌细胞 增殖 血管紧张素 diethyl-2,6-diethyl-4-fumy-1, 4-dihydropyridine-3, 5- dicarboxylate vascular smooth muscle cells proliferation angiotension Ⅱ
分类号 R541.7 [医药卫生—心血管疾病] R331.38 [医药卫生—人体生理学]
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参考文献8

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心脏杂志
2005年 第4期

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