摘要
目的探讨肾脏MMP-2、TIMP-1的表达与膜性肾病发病机制间的关系。方法把小鼠随机分为膜性肾病模型组和对照组,用免疫组织化学(SABC法)检测肾脏组织中MMP-2和TIMP-1的表达;用图像分析系统检测基底膜厚度。结果模型组和对照组肾脏中均有MMP-2、TIMP-1表达,且主要表达在肾小管上皮细胞胞浆中。模型组肾组织中MMP-2及TIMP-1表达明显强于对照组(P<0.01);模型组中MMP-2和TIMP-1表达与GBM厚度呈正相关(0.01<P<0.05),对照组MMP-2和TIMP-1表达与GBM无相关性(P>0.05)。结论在MN过程中,肾脏中MMP-2和TIMP-1参与了GBM增厚形成的机制。
[Objective] To research the relativity between mechanism of MN and the expression of MMP-2, TIMP-1 proteins. [Methods] Membranous nephropathy mice animals model were used, and the MMP-2, TIMP-1 proteins expression in kidney was assessed by immunohistochemistry and the thicknesses of GBM and TBM were measured. Then the statistical methods were used to compare and analyse the data of MN group and control group. [Results] MMP-2 and TIMP-1 proteins were expressed mainly in renal tubule of MN group and control group. The level of MMP-2 and TIMP-1 proteins in MN group increased remarkably comparing with control group. In MN group, the expressions of MMP-2 and TIMP-1 protein paralleled with the thickness of GBM, and In control group, the expression of MMP-2 and TIMP-1 protein were not relative to the thickness of GBM. [Conclusion] In MN the abnormal expressions of MMP-2 and TIMP-1 protein were relative to the mechanism of MN, and TIMP-1 maybe act an important action during the developing of MN.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2005年第16期2421-2425,共5页
China Journal of Modern Medicine
基金
泸州医学院科研基金支助课题(NO.04017)
