硝普钠对离体视上核神经元谷氨酸反应的影响被引量：2

Effects of sodium nitroprusside on glutamate-induced responses of supraoptic nucleus neurons in vitro

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摘要目的观察一氧化氮(NO)供体硝普钠(sodiumnitroprusside,SNP)对视上核(supraopticnucleus,SON)神经元谷氨酸反应的影响,以了解NO对SON细胞活动的调制作用。方法对成年大鼠下丘脑冠状切片SON神经元进行细胞内生物电记录,测定其细胞电生理参数。结果对12个SON细胞局部压力喷射微量谷氨酸,均诱发伴有膜电阻减小的去极化反应,并呈剂量和膜电位依赖性特征。SNP在67%的细胞使谷氨酸反应幅度降低(P<0.05,n=8),时程缩短(P<0.01),而在33%的细胞延长谷氨酸反应时程(P<0.05,n=4)。结论SNP对SON神经元谷氨酸反应具有抑制和增强两种不同的调制作用,提示NO对SON神经元的兴奋性突触传递可能有双向性影响。 Aim To realize the modulatory actions of nitric oxide （ NO ） on supraoptic nucleus （ SON ） neurons by observing the effects of NO donor sodium nitroprusside（SNP） on the SON neurons. Methods The cell electrophysiology properties were obtained by the intracellular recording techniques from the SON neurons in adult rat hypothalamic slices. Results Local pressure ejection of glutamate induced depolarizing responses with decrease of input resistance, concentration- and membrane potential-dependent properties in 12 SON neurons. The amplitude and duration of glutamate responses were suppressed by bath of SNP in 67% of tested cells （ P 〈 0. 05, n = 8 ）, but potentiated in the remained cells（P 〈 0.05, n = 4）. Conclusion SNP presents an inhibitory or excitatory effect on the glutamate induced responses in the SON neurons, which suggests that NO may exhibit complex and diverse interactions with glutamate, the important excitatory neurotransmitter in central nervous system.

作者黄宏平汪萌芽

机构地区皖南医学院细胞电生理研究室

出处《中国药理学通报》 CAS CSCD 北大核心 2005年第8期988-992,共5页 Chinese Pharmacological Bulletin

基金国家自然科学基金(No30270366 WMY) 安徽省人事厅人才专项基金(No2003 WMY)资助项目

关键词硝普钠视上核一氧化氮谷氨酸电生理学 sodium nitroprusside supraoptic nucleus nitric oxide glutamate electrophysiology

分类号 R-332 [医药卫生] R-322.81 [医药卫生]

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