摘要
目的:研究大鼠全肝血流阻断再灌注对肠黏膜屏障的影响。方法:60只SD大鼠,随机分成假手术组(A组)和全肝血流阻断再灌注处理组(B组),每组30只。采用阻断肝门及肝上、肝下下腔静脉20min复制大鼠全肝血流阻断再灌注模型,分别观察全肝血流阻断再灌注末(T0)、再灌注4h(T1)肠道肉眼变化,检测T0、T1门静脉血血气、门静脉血D-乳酸、TNF-α含量,观察小肠黏膜光镜及电镜下组织结构及再灌注48h大鼠生存率变化。结果:B组T0门静脉血PCO2明显升高,PO2,pH,HCO3-明显下降(P<0.05);T0,T1门静脉血D-乳酸,TNF-α及肠黏膜MDA含量明显升高(P<0.05),B组肠黏膜光镜及电镜下存在明显组织学损害,48h生存率明显低于A组(P<0.05)。结论:大鼠全肝血流阻断再灌注处理可导致肠黏膜屏障损害。
Objective To investigate the influence of treatment with total hepatic vascular exclusion and reperfusion on the intestinal barrier in rats. Methods The total hepatic vascular exclusion and reperfusion model was built after the block of hepatic portal, suprahepatic and infraheptic vena cava for 20 minutes. Sixty Sprague-Dawley rats were divided randomly into 2 groups： sham operation group （Group A, n = 30） and total hepatic vascular exclusion and reperfusion treatment group （ Group B, n=30）. Each group was subdivided randomly into 3 subgroups （n=10） according to different experiment time points as follows： at the end of the total hepatic vascular exclusion （T0） , 4 reperfusion after total hepatic vascular exclusion （T1） and the 48 h survival. Portal vein blood gas was analysed at T0. At T0 and T1 the following items were detected： the level of portal vein blood D-lactate, tumor necrosis factor-α （TNF-α）, the MDA concentration and pathologic morphology change of intestinal mucosa. Results Compared with Group A, the PCO2 at To in Group B increased while pH, PO2, and HCO3^- decreased significantly （P〈0.05）. The level of portal blood D-lactate, TNF-α and intestinal mucosa MDA at To and T1 was significantly higher （P〈0.05, or P〈0.01 ）. The histologic damage in the intestinal mucosa was observed in Group B, and the rat survival in Group B was lower than that in Group A （P〈0.05）. Conclusion The treatment with total hepatic vascular exclusion and reperfusion can damage the intestinal barrier in rats.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2005年第4期433-436,共4页
Journal of Central South University ：Medical Science
关键词
缺血再灌注
肝脏
全肝血流阻断
细菌内毒素移位
大鼠
ischemia-reperfusion
liver
total hepatic vascular exclusion
bacteria-endotoxintranslocation
rats
