摘要
采用胶原灌注-不连续密度梯度法和胶原消化-消化时差法进行了仔猪胰岛细胞分离纯化的比较性试验,结果表明胶原灌注-不连续密度梯度纯化法较胶原消化-消化时差纯化法具有明显的优越性,胰岛细胞的数量和纯度均有显著提高。
Piglet pancreas cell was digested by collagen perfusion-discontinuous density gradient centrifugation and collagen digestion-digestion time difference. Conclusions indicated collagen perfusion-discontinuous density gradient centrifugation for calf porcine pancreas cell culturing had a high yield and high purity.
出处
《黑龙江八一农垦大学学报》
2005年第4期46-48,共3页
journal of heilongjiang bayi agricultural university
关键词
仔猪
胰岛细胞
胶原灌注
胶原消化
piglet
islet cell
collagen perfusion
collagen digestion
