摘要
目的研究细胞因子干扰素γ(IFNγ)和白介素1β(IL1β)对人喉鳞癌细胞株Hep2及AMCHN8细胞粘附及浸润的生物学性状的影响。探讨细胞因子IFNγ和IL1β改变喉鳞癌细胞粘附和浸润性状的机制。方法应用细胞粘附抑制实验和快速体外浸润实验检测经细胞因子IFNγ和IL1β作用后的Hep2及AMCHN8细胞体外粘附和浸润情况。采用流式细胞仪检测经细胞因子IFNγ和IL1β作用后的喉鳞癌细胞Hep2及AMCHN8整合素α6、β4的表达。结果IFNγ和IL1β对两种喉鳞癌细胞株的生物学性状的影响各异。IFNγ可体外抑制Hep2及AMCHN8细胞的粘附和浸润。IL1β可增强AMCHN8细胞的粘附和浸润,但对Hep2细胞的粘附和浸润无影响。IFNγ和IL1β对两种喉鳞癌细胞整合素α6、β4蛋白表达有不同的调控作用。结论IFNγ和IL1β可能是通过调控不同的粘附分子进而影响喉癌细胞株的生物学性状。
Objective To study the effects of IFN-γ and IL-1β on the adhesive and invasive behaviors of human laryngeal carcinoma cell lines. Methods Adherence inhibition assay and in vitro invasive assay were used following IFN-γ and IL-1β incubated with Hep-2 and AMC-HN8 cells for 48 hours. How cytometry was used to exam the expression of the integrine α6, β4 in Hep-2 and AMC- HN8 cells. Results The effects of IFN-γ and IL-1β on Hep-2 and AMC-HN8 cell lines were different. IFN-γ could inhibit the adhesive and invasive behaviors of Hep-2 and AMC-HN8 cells. IL-1β could enhance the adhesive and invasive behaviors of AMC-HN8 cells, which had no effect on Hep-2 cells. The regulation of IFN-γ and IL-1β on Hep-2 and AMC-HN8 cell lines was differet. Conclusion It is suggested that IFN-γ and IL-1β have different effects on the adhesive and invasive behaviors of human laryngeal carcinoma cell lines through diverse adhesive molecules.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
2005年第4期217-220,224,共5页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
基金
上海市科委资助项目(No.004119017)
