摘要
目的探讨内皮抑素对喉鳞癌细胞（Hep-2）的作用及其可能的机制。方法①MTT法检测不同浓度（10—50μg/ml）的内皮抑素作用72h和30μg/ml的内皮抑素作用不同时间（24—72h）对Hep-2细胞的影响；②电镜观察Hep-2细胞超微结构的变化；③RT-PCR法检测Hep-2细胞内皮抑素作用组中Survivin基因的表达。结果①MTT检测显示，内皮抑素（20—50μg/ml）能抑制Hep-2细胞的增殖（P〈0．05，P〈0．01），具有剂量．时间依赖性；②电镜观察，Hep-2细胞内皮抑素作用组均出现凋亡改变；③RT-PCR结果，与正常Hep-2细胞对照组相比较药物作用组Survivin基因表达受到部分抑制，差异呈显著性（P〈0．01）。结论内皮抑素能抑制喉鳞癌Hep-2细胞，并具有时间-剂量依赖性，机制可能为诱导细胞凋亡。提示，内皮抑素可能通过诱导喉癌细胞本身的凋亡，对喉癌的生长与转移起到抑制作用。
Objective To evaluate the inhibitory effect and the possible mechanism of endostatin on laryngeal carcinoma cells （Hep-2）. Methods ① By means of MTF assay, the influences of endostatin with different concentrations（ 10 - 50μg/ml） and endostatin（30μg/ml） with different time（24 - 72h） on the livability of Hep- 2 cells were studied; ②The changes of Hep-2 cells＇ uhrastructures were examined by electron microscope; ③ After that, Survivin mRNA expression was measured in Hep-2 cells with endostatin（30μg/ml） by RT-PCR test. Results ①The growth of Hep-2 cells was significantly inhibited （ P 〈 0.05, P 〈 0.01 ） by endostatin （20 50μg/ml） ,what＇s more it presents dose-time-dependent; ②Apoptosis was observed in the groups of Hep-2 cells with endostatin by electron microscope;③The expression of Survivin in Hep-2 cells was partly inhibited by endostatin in the test of RT-PCR,and it was apparent difference compared with the cells without drugs（ P 〈 0.01 ）. Conclusion Endostatin can inhibit the proliferation of Hep-2 cells with time-dose-dependent and it＇s mechanism maybe induces the apoptosis. Prompt, Endostatin perhaps inhibit the growth and the transformation of laryngeal squamaous cells carcinoma through inducing the apoptosis of Hep-2 cells.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2006年第4期282-285,共4页
Journal of Harbin Medical University
基金
黑龙江省自然科学基金资助项目(D0340)
