摘要
利用AP-PCR技术对来自于海南东寨港红树林自然保护区的5种海桑属植物的遗传多态性进行了分析。由选用的46条随机引物共扩增出908个DNA片段,其中有403个DNA片段分别为不同种海桑所特有的,占总扩增条带数的44.4%,呈现出较高的多态性,它们是海桑属植物不同种间潜在的分子标记。以NT- SYSpc分析软件对这5种海桑属植物进行了聚类分析,结果表明:5种海桑属植物可聚类为3个组,其中Ⅰ组有海桑和拟海桑,Ⅱ组有无瓣海桑和海南海桑,Ⅲ组仅包括卵叶海桑一种,与传统的植物分类学研究结果略有不同,其原因有待进一步认识。
Genetic divesity of Sonneratia plants were analysed among the five species from the mangrove natural preserve district of Dongzhaigang in Hainan province. With the help of 46 random primers, 908 DNA bands were amplified through the method of arbitrarily primed polymerase chain reaction （AP PCR） ,in which there were 403 polymorphic DNA bands, about 44.4 percentage of the total DNA bands detected. They were the potential DNA markers to distinguish the different species of Sonneratia plant from each other. The clustering analysis of 5 species showed that S. hainanensis was more close to S. apetala than to S. ovata. However, according to the traditional taxology, S. hainanensis showed little difference from S. ovata than from S. apetala. It deserved a further study.
出处
《药物生物技术》
CAS
CSCD
2005年第4期232-235,共4页
Pharmaceutical Biotechnology
基金
教育部高等学校科技创新工程重大项目培育资金项目(NO704026)
