摘要
目的探讨急性胰腺炎相关腹水对在体胃肠平滑肌电生理和运动的影响,以及对肠道神经型一氧化氮合酶(nNOS)神经的影响。方法以5%牛磺胆酸钠溶液逆行注入胰胆管制作急性胰腺炎(AP)模型,24h后收集腹腔液低温离心取上清液作为胰腺炎相关腹水。24只Wistar大鼠随机分为正常对照、实验对照、腹水模型组和Nω-硝基-L-精氨酸(L-NNA)治疗组。用Maclab数据分析系统,同时记录肠运动和电信号。记录后取各组距幽门15cm的空肠全层标本,用链霉菌抗生物素蛋白-过氧化酶连接法(SP法)做nNOS免疫组化染色。结果正常组与实验对照组的空肠运动频率和平均振幅比较无明显差异;慢波峰值频率和平均振幅与正常组比较差异无统计学意义(P>0.05);腹水组与实验对照组比较运动频率和平均振幅均明显降低(P<0.05);慢波峰值频率和平均振幅与正常组比较明显降低,差异有统计学意义(P<0.05),并伴有明显节律紊乱;L-NNA治疗组慢波频率和运动频率均恢复。反映空肠收缩的运动指标和慢波平均振幅指标虽然有一定的恢复,但与腹水组比较差异无统计学意义(P>0.05)。正常肌间神经丛和黏膜下层可见nNOS阳性神经元和神经纤维,腹水组与实验对照组比较,神经元、神经纤维着色程度加深,差异有统计学意义(P<0.05)。结论胰腺炎相关腹水可引起胃肠动力抑制和胃肠电节律紊乱。胰腺炎相关腹水的这种抑制作用可能部分通过增加肠壁肌间神经丛和黏膜下层非肾上腺非胆碱能(NANC)神经中nNOS的活性来完成。
Objective To investigate the effects on the gastrointestinal motility of ascites associated with experimental acute pancreatitis and on the electrogastrointestinal arrhythmia in rats and its mechanisms. Methods AP was produced with 5 % sodium taurocholate by the method of retrograde pancreatic-injection. At 24 h after the induction of pancreatitis, the rats were sacrificed and the peritoneal exudates were aseptically collected followed by centrifugation at 4 ℃. The supernatants from the rats were combined which were designated as pancreatitis-associated ascitic fluid （PAAF）. A total of 24 Wister rats were randomly divided into 4 groups： normal group, sham group, PAAF group and LNNA plus PAAF group. We completed directly A/D change and achieved the computer real-time recording by combining the traditional recording device with Maclab/4e date recording and analysis systems. Furthermore, the nNOS located in yenteric neurons was assayed by semi-quantitatived immunohistochemical methods in rat jejunum. Results The frequency and amplitude of the slow wave （SW） of the rat jejunum were inhibited when 5 mL PAAF was injected peritoneally after 24 h （PAAF group）; while the percentage of dysrhythmias was increased （P 〈 0.05 ）, the contractive function of the intestinal smooth muscle were also inhibited more significantly than that of the sham group（ P〈 0.05）. The gray values in the yenteric neurons in rat jejunum of the PAAF group were increased obviously than that of the sham group （P〈 0.05）. Conclusion PAAF has effects on the electrogastr ointestinal arrhythmia in rats and the mechanisms may be related with the activation of nNOS in jejunum myenteric plexus during acute pancreatitis.
出处
《首都医科大学学报》
CAS
2005年第4期482-485,共4页
Journal of Capital Medical University
关键词
急性胰腺炎
腹水
胃肠动力
一氧化氮合酶
acute pancreatitis
ascitic fluid
gastrointestinal molitity
NOS
