摘要
为解决肝病救治中肝供体缺乏的问题,采用基于快速成形技术的细胞组装机,将肝细胞和海藻酸钠与明胶复合材料的共混物作为成形对象,通过细胞材料直接三维受控组装技术,实现了堆积成形具有一定三维结构和预定义孔隙的肝组织前体。常规体外培养条件下,肝细胞存活并保持生物学活性达12d之久。这一技术有望成为肝脏及其他人体组织器官人工构造的新途径。
The manufacturing of bio-artificial livers for liver transplants is a new technology with many unanswered questions. A rapid prototyping technique was developed to form a living cells/extracellular matrix （ECM） through direct three-dimensional controlled assembling. A three-dimensional liver tissue precursor structure with predefined pores composed of hepatocytes and gelatin/alginate hydrogel was formed using this technique. In vitro studies showed that the embedded hepatocytes in the structure remained viable and performed biological functions for 12 days. This technique holds potential for manufacturing bioartificial livers and other living human tissues and organs.
出处
《清华大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2005年第8期1009-1011,1031,共4页
Journal of Tsinghua University(Science and Technology)
基金
清华大学基础研究基金项目(Jc2003015)
关键词
快速成形
细胞组装机
肝细胞
三维受控组装
人工肝
rapid prototyping
cell assembler
hepatocyte
3-D controlled assembly
bioartificial liver
