摘要
本实验采用硫酸铵一次性盐析、DEAE-SepharoseFF离子交换层析和SephacrylS-200凝胶过滤层析法,分离纯化免疫初乳中的IgG,并采用SDS-PAGE电泳、单向火箭免疫电泳及试管凝集法对IgG分离物进行了定性定量检测,结果表明所得IgG纯度高,活性大。
IgG precipitated by ammonium sulfate from Immune colostrum was isolated and purified by DEAE-Sepharose FF Ion exchange chromatography and Sephacryl S-200 Gel filtration chromatography. IgG was validated by SDS-PAGE electrophoresis, one-dimension rocket immunoelectrophoresis and the methods of tube agglutination. The results demonstrated that based on high activity, the purity of IgG was significant increased.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第8期146-149,共4页
Food Science
基金
国家十五奶业重大科技专项(2002BA518A12)
江苏省十五攻关项目(BE2002302)
江苏省自然科学基金(BK2005137)
关键词
免疫初乳
IGG
分离纯化
immune colostrums
IgG
isolation and purification
