摘要
研究了聚酰胺柱层析技术吸附分离葡萄籽原花青素的效果,并对比了分级洗脱产物的清除羟自由基的能力。结果表明,聚酰胺吸附分离原花青素的适宜上样流速为0.45ml/min,上样浓度为5～8mg/ml。用80%丙酮溶液洗脱时,可获得最高的产率。在分级洗脱物中,80%和40%丙酮洗脱物的清除·OH能力最强,其次是60%浓度的丙酮洗脱液,各产物的抗氧化性均好于儿茶素。
The effects on separation and purification grape seed extracts by polyamide column chromatography, as well as the capacity of eliminating ~ OH of each segment were studied. The results showed that the suitable velocity of flow and the concentration of sample loading are 0.45 ml/min and 5-8 mg/ml respectively. The highest yield can be obtained when washing with 80% acetone. Among four washing segments, the products coming from 40% acetone and 80%acetone during washing has showed much high capacity in eliminating o OH, then came the 60% acetone. However, the capacity of eliminating · OH of each segment is better than that of catechin.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第8期113-116,共4页
Food Science
关键词
葡萄籽原花青素
聚酰胺
柱层析
化学发光法
·OH
grape seed extract （procyanidins）
polyamide
column chromatography
chemistry luminescence
·OH
