摘要
目的:探讨大杯伞菌丝体培养的最佳液体培养基及其对骨骼肌疲劳的影响。方法:采用深层培养方法,筛选出较优的碳源、氮源、无机盐,设计三因素四水平的正交试验,筛选大杯伞的最适培养基;采用脉冲式电流直接刺激蟾蜍离体腓肠肌作为疲劳模型,观察大杯伞菌丝体和任氏液对蟾蜍腓肠肌收缩能力的影响。结果:最适培养基为红糖2%、麦麸3%、磷酸二氢钾0.2%;0.8、0.3mg/ml的大杯伞菌丝体能够延长肌肉收缩下降到最大收缩幅度的90%、50%和10%的时间,与对照组相比有统计学意义,0.1mg/ml的大杯伞菌丝体能够延长肌肉收缩幅度下降到最大收缩幅度的90%的时间,与对照组相比有统计学意义(p<0.05)。结论:大杯伞菌丝体对离体骨骼肌的疲劳有延缓作用。
Object： To study the submerged culture of Clitocybe maxima mycelia and its effect on skeletal muscle fatigue. Method： Selecting the optimum carbon sources, nitrogen sources and inorganic salt to get the optimum liquid medium recipe by the orthogonal test in three factors and four levers; Isolated toad gastrocnemius stimulated by pulse electricity was regarded as fatigue model and Clitocybe maxima mycelia was compared with Ringer solution to see what influence they had on muscle contract ability. Results： The optimum liquid medium recipe was brown sugar 2%, wheat-bran 3%, KH2PO4 0.2%. Clitocybe maxima mycelia of 0.8mg/ml and 0.3mg/ml could delay the time when muscle contract extent was 90%, 50% and 10% of its maximum, while that of 0.1 mg/ml could delay the time when contract extent was 90% of its maximum（p 〈 0.05）. Conclusion： Clitocybe maxima mycelia had the function of delaying fatigue of isolated skeletal muscle.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第8期86-90,共5页
Food Science
关键词
大杯伞
深层培养
正交试验
离体腓肠肌
肌肉疲劳
Clitocybe maxima
submerged culture
orthogonal test
isolated gastrocnemius
muscle fatigue
