摘要
目的探讨细胞骨架与细胞因子在心肌肥厚过程中的作用。方法用腹主动脉缩窄的方法建立压力超负荷性心肌肥厚大鼠模型,应用放射免疫分析法测定肥厚心肌ANF(心钠素)、AngⅡ水平,RT-PCR法半定量TGF-β1 (转化生长因子β1)的表达变化,观察细胞骨架解聚剂与血管紧张素受体拮抗剂对心肌肥厚的影响。结果发现腹主动脉缩窄4周后心脏重量指数、心肌ANF、AngⅡ含量及TGF-β1的表达均明显升高,经过细胞骨架解聚剂与血管紧张素受体拮抗剂4周干预后可显著降低心脏重量指数、心肌ANF、AngⅡ含量及TGF-β1的表达。结论细胞骨架与细胞因子共同参与了压力超负荷性心肌肥厚的发生、发展,细胞骨架是细胞因子发挥作用的基础。
Objective To investigate the effect of cytoskeleton and cell factor on pressure-over-loading cardiac hypertrophy. Methods Cardiac muscle ANF and Ang Ⅱ were measured by radioimmunoassay, and ventricular transforming growth factor-beta, （ TGF-β1 ） was examined by RT-PCR after abdominal aorta constriction （ AC）. Results The levels of ANF, Ang Ⅱ and TGF-β1 mRNA and ratios of all or left heart weight to body weight were significantly increased after AC for four weeks and significantly decreased after treatment with cytoskeleton depolymerizing agent（colchicine） and AT1 recepter antagonist （valsartan）. Conclusion Cytoskeleton and cell factor play an important role in the development and progression of pressure-over-loading cardiac hypertrophy. Cytoskeleton is the fundation of the effect of cell factor.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2005年第4期320-322,共3页
Journal of Harbin Medical University
关键词
细胞骨架
细胞因子
心肌肥厚
cytoskeleton
cell factor
cardiac hypertrophy
