摘要
目的:研究氟化物控释装置(IFRD)对细菌在牙科常用合金表面粘附的影响。方法:将抛光后的Co-Cr合金和Ti合金试样各均分为8组,测定表面粗糙度后在无IFRD环境中(A、B、C、D组)和IFRD环境中(A1、B1、C1、D1组)进行细菌粘附实验,并分别于1 h、4 h、8 h、24 h取出,测定各组样本表面细菌粘附面积百分比并进行统计学检验。结果:Co-Cr合金A、B、C、D、A1、B1、C1、D1各组样本表面粗糙度分别为(0.171±0.012)μm、(0.169±0.013)μm、(0.171±0.015)μm、(0.172±0.012)μm、(0.170±0.011)μm、(0.168±0.009)μm、(0.174±0.015)μm、(0.172±0.010)μm,其细菌粘附面积比分别为(19.56±5.75)%、(30.12±6.48)%、(48.94±5.37)%、(79.28±8.19)%,(10.82±3.13)%、(13.04±4.36)%、(17.96±4.86)%、(24.38±5.89)%;钛合金A、B、C、D、A1、B1、C1、D1组表面粗糙度分别为(0.292±0.003)μm、(0.295±0.014)μm、(0.289±0.011)μm、(0.290±0.010)μm、(0.291±0.009)μm、(0.290±0.013)μm、(0.294±0.016)μm、(0.291±0.011)μm,其细菌粘附面积比分别为(24.36±5.59)%、(35.28±6.87)%、(51.45±5.82)%、(82.03±7.69)%.(16.15±4.31)%、(18.34±4.95)%、(22.59±6.90)%、(31.17±4.27)%。经统计学分析表明,无论是Ti合金还是Co-Cr合金使用IFRD后细菌粘附面积比均显著少于未使用IFRD组(P<0.01)。结论:IFRD对细菌在金属表面的粘附有明显的抑制作用,可减少菌斑的附着。
Objective： This study investigated the effect of IFRD on adhesion of oral micro- organisms on Ti alloy and Co - Cr alloy in vitro. Methods： 48 pieces Ti alloy were divided into 8 groups and their surface roughness were measured by 6AJ interference microscope. A, B, C, D groups were used for micro -organisms adhesive test without IFRD and A1 , B1 , C1, D1 groups were used for same purpose with IFRD. A standardized cell suspensions were incubated with all test samples for lh, 4h, 8h 24h at 37oC and retained cells were counted by image analysis. The 48 pieces Co - Cr alloy were treated with same procedure of Ti alloy. Results ： Roughness values and percentage area of a microscopic field covered by cells of A, B, C, D, A1, B1, C1, D1 groups of Co-Cr alloy were （0.171 ±0.012）μm.（0.169 ±0.013） μm,（0.171 ± 0.015） μm,（0. 172 ±0.012） μm,（0. 170 ±0.011） μm,（0. 168 ±0.009） μm,（0. 174 ±0.015）μm,（0. 172 ± 0.010）μm, （19.56±5.75）% ,（30. 12 ±6.48）% ,（48.94 ±5.37）% ,（79.28 ±8. 19）% , （10.82 ±3.13）%, （13.04±4.36）% ,（17.96 ±4.86）% ,（24.38 ±5.89）%. The values of A, B, C, D, Al, Bl, Cl, Dl groups of Ti alloy were （0.292 ±0.003） μm,（0.295±0.014） μm,（0.289±0.011） μm,（0.290±0.010） μm, （0.291 ±0.009） μm,（0.290±0.013） μm,（0.294±0.016） μm,（0.291 ±0.011）μm, （24.36 ±5.59）% ,（35.28 ±6.87）% ,（51.45 ±5.82）% ,（82.03 ±7.69）%, （16.15 ±4.31）% ,（18.34 ±4.95）% ,（22.59 ±6.90）% ,（31.17 ±4.27）%. There were significantly difference of percentage area of a microscopic field covered by cells between groups with and without IFRD of Ti alloy and Co - Cr alloy. Conclusions ： There was a significantly influence of IFRD on adhesion of oral micro - organisms on Ti alloy and Co - Cr alloy.
出处
《口腔医学研究》
CAS
CSCD
2005年第4期422-424,共3页
Journal of Oral Science Research
关键词
IFRD
牙科合金
细菌粘附
IFRD Dental alloy Adhesion of microorganisms
