摘要
建立HPLC法测定当归调经胶囊中芍药苷的方法。采用kromasil C18柱,流动相为甲醇-乙腈-0.025mol/L磷酸溶液(每1000ml含三乙胺1.8ml)(1.5:13.5:85);流速为1ml/min;检测波长为230nm。芍药苷在0.2050～1.0250μg范围内线性良好,平均回收率为100.91%,RSD=1.24%(n=5)。本方法不仅操作简单、准确,且重复性好,可用于当归调经胶囊的质量控制。
To establish a HPLC Determination of paeoniflorin in Angelica Tiaojing capsule method.Using kromasil C18 column,mobile phase was methanol-acetonitrile-0.025mol/L phosphate solution(per 1000ml with triethylamine 1.8ml)(1.5:13.5:85);flow rate 1ml/min;detection wavelength of 230nm.The paeoniflorin shows a good linear relationship within a range of 0.2050 ~ 1.0250μg,the average recovery was 100.91%,RSD = 1.24%(n = 5).This method is not only simple,accurate,and reproducible,and can be used for quality control of Angelica Tiaojing capsule.
出处
《贺州学院学报》
2012年第1期140-142,共3页
Journal of Hezhou University
