摘要
目的:探讨汉族人群IL-18基因启动子-607C/A位点多态性与冠心病(CHD)发生之间的关系。方法:应用序列特异性引物-聚合酶链反应技术,检测141例CHD患者和240例健康对照者IL-18基因启动子-607C/A单核苷酸多态性位点基因型,分析CHD组和对照组基因型频率和等位基因频率分布;同时进行血脂检测。结果:CHD组和健康对照组IL-18基因启动子-607C/A位点的基因型CC、CA、AA频率分别为33.3%、50.4%、16.3%和21.3%、51.3%、27.4%,等位基因C和A的频率分别为58.5%、41.5%和46.9%、53.1%。CHD组CC基因型和C等位基因频率明显高于健康对照组(χ2=4.56,P=0.03;χ2=7.49,P=0.01),具有显著性差异。结论:IL-18基因启动子-607C/A位点CC基因型人群冠心病易感性增高,C等位基因可能是CHD发生的一个遗传危险因子。
Objective To investigate the correlation between interleukin(IL)-18 gene promoter-607 A/C polymorphism and the risk of CHD in Chinese Han population.Methods The single nucleotide polymorphism at the position of interleukin(IL)-18 gene promoter-607 A/C were detected by sequence specific primer-polymerase chain reaction(SSP-PCR) in 141 patients with CHD and 240 healthy controls.The genotype and allele frequencies were analyzed in both CHD group and control group.Meanwhile,the blood lipids were measured by enzyme method.Results In CHD group and control group,the CC,CA,AA genotype frequencies of IL-18 gene promoter-607C/A were 33.3%,50.4%,16.3% and 21.3%,51.3%,27.4%,respectively;the C,A allele frequencies were 58.5%,41.5% and 46.9%,53.1%,respectively.The frequency of genotype CC and allele C at position-607 C/A of CHD group were significantly higher than those of control group(χ2=4.56,P=0.03 and χ2=7.49,P=0.01,respectively).Conclusion The CC genotype of IL-18 gene promoter-607 C/A polymorphism may contribute to the susceptibility of CHD in Chinese Han population,and allele C may be a genetic risk factor of CHD.
出处
《湖北医药学院学报》
CAS
2011年第5期471-474,479,共5页
Journal of Hubei University of Medicine
基金
湖北省教育厅重点项目(2003A012)
关键词
白细胞介素18
冠心病
单核苷酸多态性
Interleukin 18
Coronary heart disease
Single nucleotide polymorphism
