摘要
用聚合酶链反应技术（ＰＣＲ）对北京地区２４６名无关汉族健康个体载脂蛋白Ｂ基因ＸｂａＩ位点限制性片段长度多态性进行测定及其对血清脂质、载脂蛋白水平的关系进行研究。结果表明：北京汉族健康人群以Ｘ－Ｘ－纯合子为主要基因型，占９５．１２％；Ｘ＋Ｘ－杂合型仅占４．８８％，未见Ｘ＋Ｘ＋基因型，经计算符合Ｈａｒｄｙ－Ｗｅｉｎｂｅｒｇ平衡定律，具有群体代表性；少见的Ｘ＋等位基因频率仅占０．０２４，显著低于欧美白种人（Ｐ＜０．００１）；Ｘ＋Ｘ＋基因型个体平均血清总胆固醇、低密度脂蛋白－胆固醇及载脂蛋白Ｂ水平显著高于Ｘ－Ｘ－纯合型（Ｐ＜０．０５）。提示：载脂蛋白Ｂ基因ＸｂａＩ位点上Ｘ＋等位基因，在一定程度上影响体内脂质代谢，是血脂增高的内在易感因素。
Restrict fragment length polymorphism (RFLP) of the apolipoprotein B (ApoB)gene and their association with serum lipid and apolipoprotein levels were studied in Beijing Healthy Han population.A total of 246 nomolipemic subjects included in this study.Using polymerase chain reaction(PCR)techniques,two alleles were identified at XbaI locus.The results revealed that in this populaton study,the X-allele(a bsence of XbaIcutting site)was the major one;X-X-homozyous was the most frequent genotype;the distribution of genotypes was at the Hardy-Weinberg equilibrium;the frequency of rare X+allele(with XbaI cutting site,0.024)was significantly lower than that reportet in groups of Caucasian(0.445、0.454,P<0.001);X+X-genotype was found to be associated with significantly high serum levels of totol cholesteros (TC)、low density lipoprotein-cholesterol (LDL-C) and ApoB100(P<0.05);Therefore,it is suggested that XbaI RFLP of ApoB gene may be a hereditary suscepitble factor on hyperlipidemia,in someway influenced on lipid metabolism.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
1997年第5期487-492,共6页
Chinese Journal of Pathophysiology
基金
北京市自然科学基金
