摘要
目的:探讨经皮冠状动脉内支架植入术是否激活外周血单核细胞(PBMCs)中核因子κB(NF-κB)活性的表达.方法:选择NF-κB基线时呈阴性反应的稳定型心绞痛患者50例,其中单纯造影组22例,支架治疗组28例.分别于冠脉造影或支架术后4h及术后第1,2,3d留取全血抗凝标本;采用凝胶电泳迁移率实验(EMSA)测定PBMCs中NF-κB的活性.结果:支架术程中NF-κB活性呈动态变化,于术后1dNF-κB活性显著性升高呈阳性反应,术后2dNF-κB活性达峰值,术后3dNF-κB活性有下降,但仍保持在高水平表达状态.而造影组患者则没有NF-κB活性变化.结论:冠状动脉内支架术激活外周血单核细胞NF-κB活性.
AIM： To investigate the effect of coronary stenting on the activation of NF-κB in peripheral blood mononuclear cells （PBMC）. METHODS： Fifty patients were classified into two groups： 28 patients in stent-treatment group and 22 in coronary angiography （CAG）. The levels of NF-κB activity pre-procedural and during the days after stent placement were determined using electrophoresis mobility shift assay （EMSA）. RESULTS： The levels of NF-κB activity in stent-treatment group from pre-procedural to the 4th hour, 1st, 2nd and 3rd days after stent placement were （ 1.17 ± 0. 25 ） fold, （ 1.23 ± 0. 30） fold, （2. 00 ± 0. 45 ） （2.49 ± 0.46） fold and （2.25 ± 0.4） fold respectively. Sig- nificantly increased levels of NF-κB activity were seen in the 1st, 2nd and 3rd days （P 〈0.05）. No alteration of NF-κB activity was seen in CAG group. CONCLUSION： Increased level of NF-κB activation in PBMC may be involved in the stent injury of the targeted vessel wall.
出处
《第四军医大学学报》
北大核心
2005年第16期1475-1477,共3页
Journal of the Fourth Military Medical University
关键词
外周血单核细胞
NF-ΚB
支架
peripheral blood mononuclear cells
NF-kappa B
stents
