摘要
目的 探讨益气活血化痰方对慢性阻塞性肺疾病(COPD)肺功能和气道重塑的影响,并与罗红霉素进行对比.方法采用气管内注入脂多糖(LPS)和烟熏的复合方法制备COPD大鼠模型.将80只Wistar大鼠随机分为正常对照组,模型组,中药20、30、40 d组,罗红霉素20、30、40 d组.正常对照组和模型组均从第20日起每日予温开水(10 ml/kg)灌胃;中药和罗红霉素20、30、40 d组分别从第20、30、40日起每日予益气活血化痰方(0915 ml/kg)或罗红霉素(35 mg/kg)灌胃.各组于第91日测定存活大鼠的肺功能,处死动物后取肺组织,光镜下测量胶原面积.结果与正常对照组比较,模型组肺吸气阻力(Ri)显著升高(P<0.05),第0.3秒用力呼气容积与用力肺活量比值(FEV0.3%)显著降低(P<0.01);与模型组比较,中药20 d组和罗红霉素20 d组Ri显著降低,中药20 d组和罗红霉素20 d、30 d组FEV0.3%显著升高(P<0.05或P<0.01).模型组胶原面积较正常对照组显著增加(P<0.01);中药20 d组和罗红霉素20 d组胶原面积较模型组均显著减小(P<0.01和P<0.05),中药和罗红霉素30 d、40 d组胶原面积则与模型组无明显改变(P均>0.05).结论早期给予益气活血化痰方可明显改善COPD大鼠肺功能,减轻气道重塑的病理改变,其作用与罗红霉素相当.
Objective To investigate the influences of Yiqi Huoxue Huatan prescription( 益气活血化痰方) on pulmonary function and airway remodeling in rats with chronic obstructive pulmonary disease(COPD)induced by endotoxin and compare with that of roxithromycin．Methods The COPD model was induced by intratracheal injection of lipopolysaccharide(LPS) and inhalation of smoke.Eighty Wistar rats were randomly divided into eight groups ：the normal control group，model group，herb medicine 20，30，40th day groups,and roxithromycin 20，30，40th day groups ．After modeling，the normal control group and model group were given boiled water moderate in temperature( 10 ml ／kg) by gavage from the 20th day onward；the herb medicine groups and the roxithromycin groups were given Yiqi Huoxue Huatan prescription(O．915 ml ／kg)or roxithromycin( 35 mg／kg) respectively starting from the 20，30，40th day separately．Pulmonary function was tested first in all the survival rats on the 91st day and then they were sacrificed；the lungs were harvested for microscopic examination to test the average collagen area ．Results Compared with the normal control group，the inspiratory resistance( Ri )was significantly increased( P<0．05) ，and forced expiratory volume at 0．3 seconds／forced vital capacity(FEV 0．3％)was significantly declined( P<0．01) in the model group．Compared with model group，Ri was significantly declined in the 20th day groups in her bmedicine and roxithromycin groups，FEV 0．3％was significantly increased in the herb medicine 20th day group and roxithromycin 20th and 30th day groups( P<O．05 or P<O．01) ．Compared with the normal control group，the average area of collagen was significantly increased in the model group( P<O．01) ．Compared with model group，the average area of collagen was significantly reduced in the 20th day groups of herb medicine and roxithromycin( P<0．01 and P<O．05) ．Compared with the model group．the average area of collagen had no significant changes in the 30th and 40th day groups in herb medicine and roxithromycin groups (all P>O．05) ．Conclusion The herb prscription of Yiqi Huoxue Huatan given early in COPD rats can significantly improve pulmonary function，reduce the pathological changes of airway remodeling，and the effect is equal to that of roxithromycin．
出处
《中国中西医结合急救杂志》
CAS
北大核心
2009年第6期323-325,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
国家自然科学基金
关键词
肺疾病
阻塞性
慢性
益气活血化痰方
肺功能
气道重塑
chronic obstructive pulmonary disease
Yiqi Huoxue Huatan prescription
pulmonary function
airway remodeling
