摘要
按Haeffner的办法,在人肺癌细胞与人胚肺细胞中分别加入不同含量的胆固醇和卵磷脂后,测定细能的胆固醇含量、细胞核DNA含量、胞内cAMP以及以凝集反应来观察细胞的恶性程度。结果表明,提高癌细胞的胆固醇含量可使其cAMP水平增加:细胞的凝集反应减弱;四倍体细胞数减少,由原来的43降至15%;二倍体细胞数大大增加,由对照组的54%增加到79%。增加细胞的卵磷脂含量,对增加二倍体细胞数及降低四倍体细胞数也有明显影响。而在人胚肺细胞及田良肺细胞中,提高其胆周醇含量,对二倍体增加或四信体减少无明显影响。说明增加细胞胆固醇含量后,在较大程度上降低了癌细胞的恶性增殖。
The relationship between cholesterol content and proliferation of cancer cells wasstudied.When the cholesterol content of cancer cells inereased,cAMP content of thecells also increased;the agglutinability of the cells decreased;the percentage ofdiploid cells increased greatly(from 54%to 79%)and the tetraploid cells reduced ra-·107·pidly(from 43%to 15%).It was showa that the malignant proliferation of cancer cellsdecreased obviously.The cholesterol was not absorbed markedly by the V79 cell line andembryo lung celis as compared with the cancer cells when cholesterol content in themedium was increased.There were no apparent changes in the percentages of diploidcells or tetraploid cells.
作者
苗俊英
徐漱蕙
郑正炯
Miao Jun-ying(Research Center of Biotechnology,Wuhan University,Wuhan)
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1989年第2期105-107,共3页
Chinese Journal of Oncology
