摘要
通过测定谷丙转氨酶（ＧＰＴ）活力的方法，观察了牛磺熊去氧胆酸（ＴＵＤＣＡ）对ＧＰＴ的作用。发现ＴＵＤＣＡ对ＧＰＴ呈抑制作用。且随其浓度的增加，抑制作用增强，当ＴＵＤＣＡ浓度为０．８３３ｍｇ／ｍｌ时，对ＧＰＴ的抑制率达到８１％。动力学实验还表明，增加底物浓度，并不能消除ＴＵＤＣＡ对ＧＰＴ活力的抑制作用，Ｋ＿ｍ值不变，Ｖ＿ｍ值作减小，Ｋ＿ｍ／Ｖ＿ｍ值增大，呈现出非竞争性抑制作用。同时用ＣＣｌ＿４小鼠肝损伤模型，观察了口服ＴＵＤＣＡ与ＵＤＣＡ对皿清ＧＰＴ活性的影响，提示ＴＵＤＣＡ对肝脏无损害，较ＵＤＣＡ为优。
This paper deals mainly with the effects of TUDCA on activities of GPT.The studies show that TUDCA is a kind of compounds which inhibits activities of GPT.The rate of innibition is 81% at 0.833 mg/ml concentration of TUDCA.In the kineties studies,we found it impossible to relieve the inhibition depending on increasing the concentration of substrate.The value of Km/Vm in TUDCA-bearing reacion is higher that in normal.Further more,we studied the activites of TUDCA in mice.The results show that TUDCA is harmless to liver.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1995年第4期239-241,共3页
Journal of China Pharmaceutical University
关键词
牛磺熊去氧胆酸
谷丙转氨酶
酶活
中药药理学
Tauroursodeoxycholic acid(TUDCA)
Glutamic Pyruvic Transaminase(GPT)
Activity
Inhibition
