摘要
用鸡传染性贫血病病毒(CIAV)MSBI-TK5803毒株感染的MDCC-MSB_1细胞涂片为抗原,建立了检测CIAV抗体的间接免疫荧光法(HlF)。应用该HFA方法对人工感染的SPF鸡进行了检测15只1日龄SPF鸡在接种后第7、14天时均呈阴性反应,21天时2只鸡呈弱阳性反应,28天时均呈弱阳性反应,35天时呈明显阳性反应;14只40日龄SPF鸡在感染CIAV后第4天均呈阳性反应,6个月时检测仍呈阳性反应。对我国大连、沈阳和哈尔滨三地区部分鸡场鸡群进性CIAV抗体检测,结果表明我国鸡群中存在CIAV抗体。
An indirect immunofluorescent antibody technique (UFA) for the detection of antibodies against chicken infectious anemia virus (CIAV) was developed using CIAV-infected cell smears. 15 sera of 1 day-old SPF chicks inoculated with CIAV gave negative results 7 and 14 days postinoculation (PI). 2 and 15 sera of the 15 chicks gave weak positive results 21 and 28 days PI respectively. All the 15 sera of 15 chicks were detected positively 35 days PI. All the 14 sera from 14 40-day-old SPF chickens inocultated with CIAV were positive by IIFA 4 days PI,and lasted for at least 6 months. Antibodies, against CIAV were found in chicken flocks in Dalian,Shenyang and Harbin.
