摘要
用禽网状内皮组织增生病病毒（ＲＥＶ）感染ＳＰＦ鸡胚次代成纤维细胞涂片为抗原，建立了检测ＲＥＶ抗体的间接免疫荧光法（ＩＦＡ）。确定了待检血清稀释度１∶６４和兔抗鸡ＩｇＧ荧光抗体的稀释度１∶３２的工作浓度。应用该ＩＦＡ方法对人工感染ＲＥＶ的２０只３０日龄ＳＰＦ鸡进行了检测，接种后４天时均呈阴性反应，７天时８只鸡呈阳性反应，１４天２０只全部呈阳性的反应。通过对ＮＤＶ、ＩＢＤＶ、ＡＬＶ、ＭＤＶ、ＣＩＡＶ、ＡＩＶ等标准阳性血清的交叉试验，证明该方法特异性强。应用该ＩＦＡ方法对我国辽宁、山东、黑龙江省部分鸡群进行ＲＥＶ抗体检测，证明该方法特异性强、敏感性高，适于在生产中推广应用。
An indirect immunofluorescent technique for the antibody detection (IFA)of avian reticuloendoliosis virus (REV) was estabilished using chicken embryo fibroblast (CEF) cell coverslips or slides infected with REV as the antigen 1∶64 for test sera and 1∶32 for rabbit antichicken IgG were the suitable working dilution under the testing condition.Sera samples of SPF chickens (30days age)antifically infected with REV showed all positive on the 4th day、8 positive on the 7th day and all positive on the 14 day after inoculation with present method.Specificity of IFA was confirmed by the cross experiments with standard positive sera of NDV、MDV、IBDV、ALV、MDV、CIAV、and AIV Field experiment in several chicken flocks of Liaoning、Shandong and Heilongjing Province further confirmed that the IFA was specific,sensitive and suitable to be used in the practical production.
基金
国家"九五"攻关项目
黑龙江省自然科学基金
