摘要
脊髓内神经降压素（ＮＴ）在痛觉调制过程中发挥重要作用。（１）大鼠脊髓蛛网膜下腔注射ＮＴ，可显著增强电针镇痛效应，用ＡＮＴＳ预处理动物４０ｍｉｎ后，电针镇痛作用明显低于对照组。（２）脊髓蛛网膜下腔注射吗啡１０μｇ，ＮＴ的电针镇痛作用明显加强，而用１００μｇ纳洛酮预处理后的大鼠，电针１０ｍｉｎ注射ＮＴ，大鼠痛阈降低非常显著（Ｐ＜０．０１）。（３）电针大鼠双侧＂足三里＂１０ｍｉｎ，向脊髓蛛网膜下腔注射ＤｙｎＡ（３ｕｇ）和ＮＴ（２ｕｇ），注药后３０ｍｉｎ电针期间，大鼠痛阈明显升高（Ｐ＜０．０１），用ＡＤｇｎＡＳ中和脊髓释出的ＤｙｎＡ，明显降低了ＮＴ增强电针镇痛的作用。结果表明：脊髓内ＮＴ参与电针镇痛，脊髓蛛网膜下腔注射ＮＴ增强电针镇痛的作用和内源性阿片肽有关，而上述ＮＴ在电针镇痛中作用，在脊髓内至少一部分作用可能是由强啡肽Ａ中介的。
Neurotensin (NT) plays an important role in the analgesia in the spinal cord. The effects of intrathecal injection of NT, antineurotensin seruxn(ANTS),Morphinn(M),Napoxone(Nx),Dynorphin A1-13(Dyn A),and Anti- Dynorphin A serum (ADyn AS) on the pain threshold(PT) and EA analgesia in rats were investigated in this paper.The potassium iontophoresis induced tail -flick was used to measure the pain threshold.① When NT was intrathecally injected during EA analgesia, the pain threshold was dramatically increased of 142.70%～183.97 %(P<0.01).After injection of ANTS, EA analgesia only produced an increase of 9.41%～25.88 % in pain threshold, which was decreased significantly as compared with that of NRS-EA group. ② Morphin strengthens EA analgesia induced by intrarhecal injection of NT.And that NT enhanced the EA analgesic effect was antagonized by injection of Nx (100 ug).③ While injecting Dyn A (3 ug) plus NT(2 ug)during EA analgesia, the pain threshold was remarkably increase of 146 .17%～223.79%(p<0.01)as compared withthat of control groups. The pain threshold in ADyn AS-NT-EA group was only increased of 39.52%～90 .75 % , which was became weakened significantly as compared with that of NRS-NT-EA group.Our results indicated that intrathecal injection of NT enhanced theEA analgesia effect, and the EA analgesia effect induced by injectionof NT may be mediated, at least partly, by Dyn A in spinal cord of the rat.
出处
《中国行为医学科学》
CSCD
1995年第2期69-71,共3页
Chinese Journal of Behavioral Medical Science
关键词
神经降压素
电针镇痛
强啡肽A
Neurotensin Electroacupuncture analgesia.Morphin Naloxone Dynorphin A
Antiserum.
