摘要
采用超凝胶柱层析结合超滤、透析、沉淀等方法分离纯化了日本血吸虫成虫３１／３２ｋＤａ抗原。经ＳＤＳ－ＰＡＧＥ、银染和免疫印迹分析，证明免疫及生化纯度。银染及ＰＡＳ证实此抗原是一种不含糖的多肽类组分。用于ＥＬＩＳＡ和ＩＨＡ诊断日本血吸虫病，与ＳＥＡ同样敏感，而特异性明显较优。
Major diagnostic proteins of 31/32 kDa were purified from soluble adult worm homogenate of Schistosoma japonicum using AcA ultragel chromatography and additional treatments. The purity of the isolated antigenic proteins ( Sj 31/32) was demonstrated by SDS PAGE and Western blotting technique. The antigens showed a negative reaction when stained by PAS and remained active after treatment with sodium periodate, moving toward anode in the electrophoretic field.These purified antigens were used for detecting the specific antibody in sera from patients with schistosomiasis japonica by ELISA and IHA. As compared with SEA, Sj 31/32 kDa was found as sensitive as but much more specific than SEA in immunodiagnosis.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1995年第1期25-31,共7页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
日本血吸血
31/32kDa抗原
免疫诊断
血吸虫病
Schistosoma japonicum , 31/32 kDa antigen, purification, chromatography,immunodiagnosis
