摘要
采用中和试验比较了草鱼出血病病毒ＧＣＨＶ８７３的１１个多肽的免疫原性，确定了主要中和抗原。纯化的单个病毒多肽免疫家兔获得抗血清，多肽ＶＰ＿１、ＶＰ＿５、ＶＰ＿６和ＶＰ＿９的抗血清具有中和效价，而ＶＰ＿２、ＶＰ＿３、ＶＰ＿４、ＶＰ＿７、ＶＰ＿８、ＶＰ＿（１０）和ＶＰ＿（１１）则不能诱生中和抗体。其中ＶＰ＿６的抗血清中和效价最高，因此，ＶＰ＿６极可能是病毒多肽中的主要中和抗原。
he immunogenicity of the 11 polypeptides of GCHV873 were commnd by using neutralizationtest for the determination of the major protective antigen.The viral polypeptides labelled with FITCwere analyzed on a 10%polyacrylamide gel.The individual polypeptides were retrieved from the gelby electric elution,and were used for immunizing rabbits to prepare antisera.Each anti-polypeptide antiserum was titrated in a neutralization test.The neutralization titres of the andsera indicated that theneutralizing activity of anti VP_6 antiserum was thehighest among them,while the VP_2,VP_3,VP_4,VP_7,VP_8,VP_(10) and VP_(11) could not induce neutralizing antibodies.Therefore, VP_6 may be a major neutraliring antigen.
出处
《中国病毒学》
CSCD
1995年第2期166-168,共3页
Virologica Sinica
关键词
草鱼出血病
多肽
病毒
免疫原性
Neutralzation test,GCHV,Polypeptides,Neutralization titre,Neutralizing antigen
