摘要
以长春新碱诱导的耐药细胞Ｋ５６２／ＶＣＲ和阿霉素诱导的耐药细胞Ｋ５６２／ＤＯＸ及敏感细胞Ｋ５６２作为检测对象，采用逆转录多聚酶链反应法，并且以β２－微球蛋白（β２ｍ）基因作为内参照，检测和比较了上述细胞的多药耐药基因ｍｄｒ１表达。经过３０个循环扩增，耐药细胞检测出ｍｄｒ１和β２ｍ基因的扩增产物，而敏感细胞无ｍｄｒ１扩增产物。结果提示，本方法可用以区分耐药和敏感细胞，有希望成为临床个体化疗敏感性预测的重要指标之一。
A highly sensitive,specific assay for measuring the expression of mdr 1 gene in tumor cells,based on the reverse transcription-polymerase chain reaction (RT-PCR),was established.The expression of mdr 1 gene of the drug resistant cell lines K5 62/VCR,K562/DOX,induced by vincristine and doxorubicin respectively,and the sensitive cell line K562 was examined by RT-PCR.It was shown that through 30 cycles of amplification,the mdr 1 product could be detected in the resistant cell lines K562/VCR and K562/DOX,but not in the sensitive cell line K562.The findings suggested that RT-PCR assay could be used to distinguish drug-resistunt cells from sensitive cells and might be considered as one of the important parameters in predicting the sensitivity of anticancer drugs for individual patients clinically.
基金
国家"八五"攻关基金
浙江省自然科学基金
关键词
聚酶链反应
抗药性
基因
长春新碱
阿霉素
Multidrug resistance gene
Reverse transcription-polymerase chain reaction
Human leukemia cell line
Tumor cells,cultured
