摘要
从大豆栽培品种Ｕｎｉｏｎ（Ｇ．ｍａｘ）基因组ｐＵＣ１８质粒文库中，以基因组ＤＮＡ为探针，筛选出一个重复序列家族。序列分析表明，此重复序列的重复单位为９１ｂｐ，拷贝数约为１０￣５，其序列约占基因组ＤＮＡ的０．９％。基因组ＤＮＡ不同限制酶片段Ｓｏｕｔｈｅｒｎ杂交分析和染色体原位杂交分析表明此重复序列主要以串联方式集中分布在Ｍ２和Ｍ１１号染色体的臂上，而另外一些则散布于整个Ｍ１２和Ｓｍ７号染色体上。以该序列为探针片大豆属不同亚属１３个种的１８个品系的Ｓｏｕｔｈｅｒｎ杂交结果表明，此重复序列为Ｓｏｊａ亚属所特有。这一Ｓｏｉａ亚属特异重复序列的发现，从另一个角度支持应把Ｓｏｊａ亚属的３个种Ｇ．ｓｏｊａ、Ｇ．ｇｒａｃｉｌｌｉｓ、Ｇ．ｍａｘ划分为一个种的观点。
One satellite DNA family,cloned from Glycine max,was sequenced and chracterized. The unit of the repetitive sequences was 91 bp。They concentrated on the arms of chromosomes M2 and M11,and spreaded on whole chromosomes Sm7 and M12.Southern blot analysis with 18 acessions,belonged to subgenus Glycine and subgenus Soj a of genus Glycine,showed that the repetitive sequences were specific to subgenus Soj a. It was subgenus specific repetitive sequence,This result, from another aspact,supported the idea that the three species in subgenus Soj a should be considered as one species.
基金
中国科学院重大项目
关键词
大豆
重复序列
克隆
特性分析
染色体
定位
Satellite DNA,Glycine max,Subgenus specific repetitive sequences.
