摘要
用小鼠神经母细胞瘤细胞无血清培养建立神经细胞老化实验模型。以流式细胞光度术观察ＤｙｎＡ（１－８）对老化过程中神经细胞的细胞周期和细胞总蛋白的影响。结果发现ＤｙｎＡ（１－８）可延缓细胞老化的细胞周期和细胞总蛋白的变化。提示ＤｙｎＡ（１－８）是通过使细胞周期和细胞总蛋白发生变化以发挥其延缓神经细胞老化的作用。
Serum-free culture of mouse neuroblastoma cells was usetd
as the experimentalmodel for the study of neuronal aging , with flow
cytometry of cell cycle , DNA and total cellularprotein as the
indices of neuronal aging.After addition of dynorphin
A(1-8)10-7mol·L-1 into theculture medium, the following general
tendencies were obtained:(1) The number of S and G2+Mphase cells was
increased and the number of G1 phase cells decreased.(2) The total
cellular protein inaged experimental neural cells decreased. The
results implies that Dyn A( 1-8) Produced effects oncell cycle, DNA
and total cellular protein in the delaying neuronal aging. The
relationbetween Dyn A(1-8) and neuronal aging mierits further
investigation.
出处
《药学学报》
CAS
CSCD
北大核心
1995年第9期646-650,共5页
Acta Pharmaceutica Sinica
基金
上海高等院校科学发展基金
河北省自然科学基金
关键词
细胞老化
细胞周期
细胞总蛋白
强啡肽
神经细胞
Dyn A( 1-8)
Neuronal aging
Cell cycle
Total
cellular protein
Flow cytometry
