摘要
选用４２只健康ＡＡ鸡，分３组（每组１４只）进行药物动力学研究：静注、肌注及内服的剂量均为１０ｍｇ／ｋｇ。用二氯甲烷提取血浆中的药物，反相高效液相色谱法测定血浆氟哌酸的浓度，测定时以吡哌酸为内标。所得药物一时间数据用ＭＣＰＫＰ计算机程序处理，静注给药拟合二室开放模型，主要动力学参数是：ｔ１／２α０．１４±０．０８ｈ，ｔ１／２β３．６５±１．０３ｈ，Ｋｅ０．８８±０．４０ｈ－１，Ｖｄ３．８１±０．９８Ｌ／ｋｇ，ＡＵＣ１４．１４±３．２９μｇ／ｍｌ．ｈ。内服和肌注给药均适合具有一级吸收二室开放模型，肌注的主要动力学参数是：ｔ１／２ｋａ０．２２±０．１０ｈ，ｔ１／２α１．０３±０．６５ｈ，ｔ１／２β６．８７±２．８６ｈ，Ｋｅ０．２８±０．１０ｈ－１，ＡＵＣ９．８６±２．８８μｇ／ｍｌ．ｈ，Ｆ６９．７８％。内服的主要动力学参数是：ｔ１／２ｋａ０．６９±０．２３ｈ，ｔ１／２α１．７６±０．８９ｈ，ｔ１／２β１０．４３±３．２２ｈ，Ｋｅ０．２１±０．０５ｈ－１，ＡＵＣ８．５６±１．３６μｇ／ｍｌ．ｈ，Ｆ６０．５２％。鸡静注、肌注、内服氟哌酸的药动学特征是吸收较快，在体内分布广泛、半衰期较长。
The pharmacokinetics of norfloxacin was investigated at 10 mg/kg dosing rate fol-lowing single intravenous, intramuscular and oral administration in 42 healtliy broilers.Norfloxacin in plasma was extracted with bichlormethane and its concentrations were de-termined using high Performance liquid chromatography (HPLC). The pipemidic acid wasas an internal standard. The drug concentration-time data in Plasina were analysed with MCPKP program. The concentration-time data of norfloxacin in plasma after intravenousadministration were fitted to a two-compartment open model. The main parameters wereas follows: t1/2a 0.14±0.08 h, t1/2β 3.65±1.03 h, Ke 0.88±0.40 h-1, Vd 3.81±0. 98 1/kg, AUC 14.14±3.29 ug/ml·h. The Pharmiacokinetics of the drug after intra-inuscular administration were described by a two-compartment open model with first-orderabsorption. The main parameters were as follows: t1/2Ka 0.22±0.10 h, t1/2α 1.03±0.65h, t1/2β 6.87±2.86 h, Ke 0.28±0.10 h-1, AUC 9.86±2.88 ug/ml·h, F 69.73%.Pharmacokinetics of norfloxacin following oral administration were described by a two-compartment open model with first-order absorption. The main paraineters were as fol-lows: t1/2 ka 0.69±0.23 h, t1/2α 1.76±0.89 h, t1/2β10.43±3.22 h, Ke 0.21±0.05h-1, AUC 8.56±1.36 ug/ml·h, F 60.520/0. The multiple dosage regimen were recom-mended according to the parameters of norfloxacin following oral administration.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1995年第1期65-70,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
广东省自然科学基金
