摘要
用海藻酸钠、二醋酸纤维、明胶分别作为固定化果胶酶的载体，研究比较载体的用量、交联剂的浓度、用酶量、制备的ｐＨ。结果表明，选择１５％浓度的明胶为载体，５％浓度的戊二醛为交联剂，用酶量５％，在ｐＨ３．５条件下制备固定化果胶酶，其酶活力回收率可达６７．７４％，重复回收使用１０次后，酶活力还可保留８０％以上，是果胶酶固定化的一种较好的方法。
Sodium alginate，diacetate fibre and gelatin were used as carriers respec-tively for immobilization of pectinase.Acomparison was made among the preparing con-ditions，i.e. the dosage of carrier，the concentration of cross linking agent，the dosageof pectinase and pH. The results showed that the recovery ratio of pectinase activity wasup to 67.74％when the immobilized pectinase was prepared with 15％gelatin as carri-er，5％glutaric acid as cross linking agent and 5％pectinase at pH 3.5，and the enzymeactivity retained more 80％after recovering 10 times repeatedly．This suggests that gela-tinization is a better method for immobilization of pectinase.
关键词
果胶酶
固定化
载体
Pectinase，Immobilization，Carrier
