摘要
目的研究大鼠间脑是否有向腰骶髓的直接投射，为进一步研究间脑对骶副交感核的直接支配提供形态学依据。方法应用ＨＲＰ逆行追踪法，将５％ＷＧＡ－ＨＲＰ注入Ｗｉｓｔａｒ大鼠腰６和骶１两段，动物存活力７２ｈ，灌注后取脑，冰冻切片，作联苯胺兰色反应，观察间脑ＨＲＰ标记细胞分布情况。结果室旁核、下丘脑外侧区、室核、前核、弓状核、交叉上核、背内侧核的外侧部和后核、丘脑束旁核、束旁下核及腹内侧核大细胞部，底丘脑未定带及ＦｏｒｅｌＨ２区，均有ＨＲＰ标记细胞分布，同侧优势。结论大鼠间脑可向腰骶髓发出直接投射纤维。）
Objective To observe whether having the direct projections from the diencephalon to lumbosacral Spinal cord in rat and to put forward the morphological evidence to further research that diencephalon directly modulates the sacral parasympathetic nucleus.Method Using HRP retrograde tracing method, 5% WGA-HRP was injected into one side of 6th lumhara and Ist sacral segmentes in 20 rats,72 hours later, the rats perfused were killed and their brains were took out, diencephalon was sectioned by freezing. Sections were dyed with TMB method.Results The labeled neurous, in both side of the diencephalon but ipsilateral prominece, were found in the following nuclei; paraventricular nucleus, lateral area of hypothalamus,periventricular nucleus, anterior nucleus,arcuate nucleus, suprachiasmatic nucleus, lateral part of dorsomedial nucleus and posterio nucleus of the hypothalamus, parafascicular nucleus,subparfascicular nucleus and magnocellular part of ventrimedial nucleus of thalamus, Zona incerta and the Foral H2 field in subthatamus.Conclusion There are direct projectiones from diencephalon to lumbarosacral spinal cord in rat.
出处
《新乡医学院学报》
CAS
1995年第2期99-101,共3页
Journal of Xinxiang Medical University
关键词
大鼠
间脑
脊髓
纤维联系
辣根过氧化物酶
rat, diencephalon
spina cord
fiberous connection
hors radish peroxidese
