摘要
以球孢白僵菌（Ｂｅａｕｖｅｒｉａｂａｓｓｉａｎａ）１３１６为出发菌株，通过２０ｍｉｎ和３０ｍｉｎ的紫外线交替诱变分生孢子，采用透明圈法初筛和摇瓶培养复筛的方法，得到一突变株ＣＨ－１３１６。和原始菌株相比，该突变株的几丁质酶活力提高了近３倍，经传代培养，该菌株的几丁质酶高产特性能稳定遗传。
he conidia of Beauveria bassiana 1316 were mutagenized by UV treatmentfor 20 minutes and 30 minutes alternately.A high chitinase-producing mutant strain wasobtained by screening with the method of transparent zones and chitinase activity measure-ment of culture filtrate. The chitinase activity of the mutant was raised 3 times as comparedwith the origiral strain,The high chitinase-producing ability of the mutant could be inhe-rited after several subcultures.
出处
《微生物学通报》
CAS
CSCD
北大核心
1995年第4期197-200,共4页
Microbiology China
基金
国家自然科学基金
关键词
球孢白僵菌
几丁质酶
高产菌株
诱变
发酵
Beauveria bassiana, high chitinase-producing strain,mutagenesis
