摘要
从不同地区采集并分离到卵孢白僵菌菌株１７个，其中菌株Ｂｅａｕｖｅｒｉａｔｅｎｅｌｌａ２４一１对大黑鳃金龟幼虫的室内致病力高达７３．３％。通过单孢分离、紫外线照射分生孢子和原生质体的诱变方法，获得了比出发菌株Ｂ．ｔｅｎｅｌｌａ２４一１产孢量高２倍以上，毒力提高０．５４～１．７９倍的优良菌株４个。
eventeen
isolates
of Beauveria tenella were obtaincd from different areas of China,Of thesefield collected isolates
B. tenella Isolate 24-1 had the highest infection rate (73.3%)onHototrichia paralla
[Col.:Scarabacidae]in the laboratory bass.A series of artificial sclectionwere applied to obtain
superior strain s of this isolate for higher sporulation rate andvirulence,Through single-spore
isolation and ultraviolet radiation on the conidia andprotoplasm,two new strains from the
single-spore isolation and one mutant strain from theradiation tests were obtaincd. Compared
with the original lsolate 24-1, the sporulation rateof the new strains were increased by two folds
and the virulence against H.paralla inereasedby almost 50%.
关键词
卵孢白僵菌
蛴螬
菌种
选育
防治
Beauveria tenella
holotrichia paralla strain selection microbial control
