摘要
在φ１０ｍｍ×１５００ｍｍ色谱柱上，同时进行蔗糖酶促水解与液相色谱分离，制备葡萄糖和果糖．Ｃａ２＋型００３×７离子交换树脂为固定相，稀酶溶液为流动相，系统研究了酶浓度、流速、进料体积、进料浓度对过程的影响，建立了描述过程的数学模型并进行了数值求解．结果表明，采用该耦合方式不仅显著加快水解速率，而且可直接收集到富葡萄糖浆与富果糖浆，减少了下游进一步分离操作的强度与费用．理论计算值与实验值在低浓度下吻合良好。
A biochemical reaction and separation took place simultaneously in a φ10×1500 chromatographic column to convert sucrose to glucose and fructose using the enzyme invertase.The column was packed with 003×7 calcium form ion exchange resin. Factors affecting the performance of the hybrid process such as enzyme-eluent concentration, eluent flowrate,pulse volume, feed concentration were investigated systematically. A mathematical model was established and solved numerically to simulate the hybrid process. The results show that not only the reaction rate is increased, but also glucose-rich and fructose-rich products are directly obtained so that the capital costs in the downstream processing can be reduced. Theoretical predictions are in good agreement with experimental data at low sucrose concentrations.
出处
《天津大学学报》
EI
CAS
CSCD
1995年第2期175-180,共6页
Journal of Tianjin University(Science and Technology)
基金
国家教委优秀年轻教师基金
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