摘要
用＾３Ｈ－ＴｄＲ掺入法检测由ＰＨＡ诱导的正常人淋巴细胞转化率，实验中分别加入进展期胃癌患者的脾静脉血清、脾细胞培养上清及根治术前、后的外周静脉血清。结果：（１）进展期胃癌患者脾静脉血清较自身术前外周静脉血清具有更高的免疫抑制活性。（２）患者脾细胞培养上清有显著的免疫抑制活性。（３）联合脾切除后７－１０天，外周静脉血清中免疫抑制活性显著降低，结果提示进展勘有癌患者的脾脏产生了免疫抑制因子。
The serum from splenic venous blood and the supernatant from spleen cell cultures as well as the serum from pre-and-postoperative peripheral blood in advanced gastric cancer patients were respectively added into the microtest plates in which the PHA-induced transformation of normal lymphocyte was assayed by ~3H-TdR incorporation test.The results of experiments are as follow: (1)Compared with the self-control serum from preoperative peripheral blood,the serum from venous kind of splenic origin blood of advanced gastric cancer patients had greater immunosuppressive activity.(2)The supernatant from spleen cell culture from advanced gastric cancer patients possessed intensive immunosuppressive activity.(3)The suppressive activity of serum from peripheral blood was significantly decreased 7 to 10 days after radical gastrectomy combined with splenectomy.The above results suggest that the spleen of patients with advanced gastric cancer can release some immunosuppressive factors and may participate in the forming of systamie immunosuppressive status, and radical gastrectomy combined with splenectomy can contribute to exhaustin,the serum immunosuppressive activity of the patients during the early postoperatively period.
出处
《苏州医学院学报》
1995年第6期1026-1028,1201,共3页
Acta Academiae Medicinae Suzhou
关键词
进展期
胃癌
脾脏
免疫抑制活性
advanced gastric cancer
spleen
immunosuppressive activity
gastrectomy combined with splenectomy
