摘要
本文报道２０例原发不育妇女、２５例习惯性流产妇女以及１１例正常生育妇女血清作ＢＷＷ培养基添加剂分别对昆明小白鼠的８１３个、１０８５个和３８１个２—细胞鼠胚进行体外培养的比较研究。结果显示：各组７２ｈ囊胚形成百分率分别为４８．７０±１３．５０％，３７．３２±２０．３５％和５９．０５±７．３３％。不育组、习惯性流产组囊胚形成率与正常生育组囊胚形成百分率经统计学处理分别为Ｐ＜０．０５，Ｐ＜０．０２，均有显著性差异。这些结果提示：部分不育与习惯性流产妇女血清中可能存在有抑制早期胚胎发育的因子，因此检测血清中鼠胚生长抑制因子，有助于分析免疫性妊娠失败的病因。
Serum was added to 2-cell mouse embryo of Kunming mouse in vitro culture were undertaken by using BWW medium. These sera were collected from 20 patients with in fertility and 25 patients with recurrent spontaneous abortion and 11 normal fertile women. 813 of the infertile group, 1085 of recurrent spontaneous abortions group and 381 of the control group were incubated in 5% CO2 in air at 37℃ for 72 h. Development to the blastocysts stage was observed. The percentage 2-cell embryos that progressed to blastocysts in three groups were respectively 48. 70 ±13. 50、 37. 32±20. 35%、59. 05±7. 33%. A significant different was found between the infertile, recurrent spontaneous abortion groups and the control group (t=3. 378 P<0. 05, t=3. 4143 P<0. 02). A serum sample was defined as toxic factor if <50% of 2-cell embryos progressed to the blastocyst stage by 72 h. 44% serum samples of infertile group, 56% serum samples of recurrent spontaneous abortion group were defined as toxic. These results suggested the presence of serum factors that are toxic to early embryonic development may play a role in the pathogenesis of infertility and recurrent spontaneous abortion.
出处
《生殖与避孕》
CAS
CSCD
北大核心
1995年第5期337-341,共5页
Reproduction and Contraception
关键词
不育
习惯性流产
2-细胞鼠胚
囊胚
体外培养
Infertility, Recurrent spontaneous abortion, 2-cell mouse embryos, Blastocyst,In vitro culture
