摘要
把黑曲霉糖化酶cDNA连同酵母α因子启动子及其分泌序列,通过转化整合到酿酒酵母染色体DNA上,获得了整合型的分解淀粉酵母转化子。Southern印迹分析证明了糖化酶cDNA对酵母染色体DNA的整入。整合型转化子在以可溶性淀粉为碳源的培养基中分泌糖化酶活力达2.5u/ml,在非选择性培养基中连续转移10次,糖化酶分泌活力稳定不变。
Starch digestible S-integrants were constructed by integrative transformation of a linear YIP plasmid carrying A. niger glucoamyase cDNA under the control of the MFa1 promoter and its prepro signal and the S sequence of the Ty element from yeast. The integration of glucoamylase cDNA to Saccharomyces cerevisiae genome was identified by Southern analysis. The secreted glucoamylase activity of integrants in the medium with soluble starch as carbon source reached 2.5u/ml. After ten times successive transfers in nonselective medium the activity of secreted glucoamylase of integrant was approximately at its original level.
出处
《生物工程学报》
CAS
CSCD
北大核心
1995年第4期321-324,共4页
Chinese Journal of Biotechnology
关键词
酿酒酵母
糖化酶
整合
黑曲霉
Glucoamylase
Saccharomyces cerevisiae
integration
stable expression
