摘要
采用银杏果实为原料诱导得到颗粒状愈伤组织,在MS培养液中筛选克隆细胞,单细胞在MS+3·0mg/LNAA+1·0mg/LKT中培养。细胞悬浮液与3%的海藻酸钠混合后滴入2%CaCl2MS,制成小球凝胶。固定化细胞在MS+3·0mg/LNAA+1·0mg/LKT+10mg/L肌醇的培养基中悬浮培养25d,黄酮生物合成量为85·63mg/L,克隆细胞增长速度提高24·45倍。
In this paper, ginkgo granular callus was obtained and the cloning cells were cloned in the MS medium. 3.0 mg/L NAA and 1.0 mg/L KT were added in MS medium added for the single-cells culture. The suspended solution of cells was mixed with 3% sodium alginate solution, and dripped into MS containing 2% CaCl2 to form small gelling balls. The immobilized cells were suspended and cultured for 25 days on the medium of MS＋3.0mgL NAA ＋1.0mg/L KT ＋ 10mg/L inositol. The amount of biosynthetic flavonoids was 85.63% mg/L and the growth rates of the cloning-cells reached 24.45 times.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2005年第7期45-48,共4页
Food and Fermentation Industries
