期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

阿霉素肾病大鼠podocinmRNA和蛋白的表达 被引量：1

Expressions of podocin mRNA and protein in adriamycin-induced nephropathic rats
暂未订购
导出
摘要 目的观察阿霉素肾病大鼠肾小球上皮细胞足突蛋白podocin mRNA和蛋白的表达。方法肾病组予阿霉素5mg/kg尾静脉注射一次,正常对照组予等量生理盐水尾静脉注射,注射后2周24h尿蛋白定量>30mg为肾病模型成立。观察4周后留24h尿行蛋白定量及血生化检查,并应用半定量RT-PCR和印迹法(Western blotting)测定podocinmRNA和蛋白质的表达。结果正常对照组大鼠podocin蛋白表达的相对量是5·26±1·19,而肾病组大鼠podocin蛋白基本不表达;正常对照组、肾病组podocin分子mRNA的相对值分别为1·06±0·19、1·96±0·98,肾病组大鼠podocinmRNA的表达与正常对照组相比显著上调(P<0·05)。结论阿霉素肾病大鼠蛋白尿的产生可能与podocin蛋白表达的减少有关。 Objective To investigate the expressions of podocin mRNA and protein in adriamycin-induced nephropathic rats. Methods ADR （5 mg/kg） was intravenously injected to the nephropathy group, while Saline was intravenously injected to the normal control group. Two weeks after the injection, the rats with 24 h protein excretion 〉 30 mg were treated as nephropathic model. The expressions of podocin mRNA and protein were measured with the semi-quantity RT-PCR and western blotting. Results The relative value of podocin protein in normal control group was 5.26 ± 1.19 and there was no expression of podocin protein in the nephropathy group. The relative values of podocin mRNA in the two groups （ normal control, nephropathy groups） were 1.06 ± 0. 19, 1.96 ± 0. 98, respectively. The expressions of podocin mRNA in the nephropathy group increased significantly in comparison with that in the normal control group （ P 〈 0. 05 ）. Conclusion The production of proteinuria in adriamycin-induced nephropathic rats may be related with the reduction of podocin protein expression.
作者 邓芳 鹿玲 王明丽 李艳秋
机构地区 安徽医科大学第一附属医院儿科
出处 《安徽医科大学学报》 CAS 北大核心 2005年第4期311-313,共3页 Acta Universitatis Medicinalis Anhui
关键词 大鼠 肾病综合征 疾病模型 动物 基因表达 rats nephrotic syndrome disease models, animal gene expression
分类号 R692 [医药卫生—泌尿科学] R341 [医药卫生—基础医学]
  • 相关文献

参考文献8

  • 1魏泓.医学实验动物学[M].成都:四川科学技术出版社,1997..
  • 2Wickelgren I. First components found for new kidney filter.Science,1999; 286(5438):225-6.
  • 3Boute N,Gribouval O,Roselli S et al. NPHS2, encoding the glomerular protein podocin, is mutated in autosomal recessive steroid-resistant nephrotic syndrome. Nat Genet,2000;24(4):349～54.
  • 4Kaplan JM,Kim SH,North KN et al. Mutations in ACTN4, encoding alpha-actinin-4, cause familial focal segmental glomerulosclerosis. Nat Genet,2000;24(3):251～6.
  • 5Luimula P, Sandstrom N, Novikov D et al. Podocyte-associated molecules in puromycin aminonucleoside nephrosis of the rat. Lab Invest,2002; 82(6):713～8.
  • 6Bertani T, Poggi A, Pozzoni R et al. Adriamycin-induced nephrotic syndrome in rats: sequence of pathologic events. Lab Invest, 1982;46(1):16～23.
  • 7Fincham VJ, James M, Frame MC.Active ERK/MAP kinase is targeted to newly forming cell-matrix adhesions by integrin engagement and v-Src.EMBO J,2000;19(12):2911-23.
  • 8Steven P,Yvan RB,Robert F et al.Correlation between protein and mRNA abundance in yeast .Mol Cell Biol,1999;19(3):1720-30.

共引文献7

同被引文献11

  • 1舒毅,钟历勇,石立.褪黑素、维生素E和还原型谷胱甘肽对四氧嘧啶糖尿病大鼠氧化应激影响的比较研究[J].东南大学学报（医学版）,2006,25(2):88-92. 被引量：12
  • 2Nakatsue T,Koike H,Han G D,et al.Nephrin and podocin dissociate at the onset of proteinuria in experimental membranous nephropathy[J].Kidney Int,2005,67(6):2239-53.
  • 3Guan N,Ding J,Deng J,et al.Key molecular events in puromycin aminonucleoside nephrosis rats[J].Pathol Int,2004,54(9):703-11.
  • 4Niwa T,Naito C,Mawjood A H,et al.Increased glutathionyl hemoglobin in diabetes mellitus and hyperlipidemia demonstrated by liquid chromatography/electrospray ionization-mass spectrometry[J].Clin Chem,2000,46(1):82-8.
  • 5Mimnaugh E G,Trush M A,Bhatnagar M,et al.Enhancement of reactive oxygendependent mitochondrial membrane lipid peroxidation by the anticancer adrug adriamycin[J].Biochem pharmacol,1985,34(6):847-56.
  • 6Kestila M,Lenkkeri U,Mannikko M,et al.Positionally cloned gene for a novel glomerular protein nephrin is mutated in congenital nephritic syndrome[J].Mol Cell,1998,1(4):575-82.
  • 7Boute N,Gribouval O,Roselli S,et al.NPHS2,encoding the glomerular protein podoc in,is mutated in autosomal recessive steroid resistant nephmtic syndrome[J].Nat Genet,2000,24(4):349-54.
  • 8Shih N Y,Li J,Karpitskii V,et al.Congential nephritic ayndrome in mice lacking CD2-associated protein[J].Science,1999,286(5438):312-5.
  • 9Kaplan J M.Kim S H.North K N,et al.Mutations in ACTN4,encoding actinin-4,cause familial focal segmental glomendosderosis[J].Nat Genet,2000,24(3):251-6.
  • 10Schwarz K,Simons M,Reiser J,et al.Podocin,a raft-associated component of the glomerular slit diaphragm,interacts with CD2AP and nephrin[J].Clin Invest,2001,108(11):1621-9.

引证文献1

二级引证文献4

安徽医科大学学报
2005年 第4期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部