摘要
以砂培法研究了不同浓度(5、10、30、50μmol·L-1)Cd胁迫对萌发大豆种子中活性氧代谢的影响。结果表明,低浓度(5μmol·L-1)Cd处理下,在萌发初期(1～3d)大豆种子中O2-产生速率、H2O2含量与对照无显著差异,萌发第3d大豆种子中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性明显高于对照,萌发第4～6dCd对SOD、CAT活性产生抑制作用,同时MAD含量和种子外渗电导率显著升高;高浓度(10、30、50μmol·L-1)Cd处理下,萌发2～6d种子中O2-产生速率、H2O2含量、MDA含量和外渗电导率显著增加,SOD和CAT活性明显下降;Cd对萌发大豆种子中过氧化物酶(POD)具有激活效应,且随着Cd浓度的增加激活效应逐渐增强。大豆种子萌发受Cd胁迫伤害过程中,活性氧代谢失衡,从而造成膜脂过氧化加剧。
Effect of active oxygen metabolism in germinated soybean seeds under cadmium stress were studied with pot culture experiment. The results showed that compared with the control, under the treatment of lower concentration cadmium, there were no significant differences in generative rate of superoxide radical （O2-）, H202 content in germinated soybean seeds during the beginning period （1-3 d）. Under the treatment of lower concentration cadmium, the activities of superoxide dismutase （SOD）, catalase （CAT） were increased significantly on the 3rd day, while, they decreased and malondialdehyde （MDA） content and membrane electrolytic leakages increased significantly after four days. Compared with the control, under the treatment of higher concentration （10, 30, 50 μmol·L^-1） cadmium, the generative rate of O2^-, H2O2 content, MDA content, membrane electrolytic leakages increased significantly and the activities of SOD, CAT decreased during 2～6 d. Cd^2＋ stimulated peroxidase （POD）activity and the activity increased gradually with the increase of Cd^2＋ concentrations. Injury effects of Cd^2＋ stress on active oxygen metabolism in germinated soybean seeds could aggravate the membrane lipid peroxidation.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2005年第4期670-673,共4页
Journal of Agro-Environment Science
基金
国家自然科学基金项目(30370862)
吉林省科技厅项目(20000204-01-04)
关键词
镉
大豆种子
活性氧代谢
抗氧化酶
cadmium
soybean seeds
metabolism of active oxygen
antioxidant enzymes
