摘要
目的:研究携带小鼠IL-10基因的重组腺病毒表达载体修饰的CD4+T细胞对哮喘小鼠气道NF-κB表达的影响,从而探讨IL-10治疗哮喘的可能分子机制。方法:BALB小鼠随机分成6组,正常对照组(A组,),哮喘模型组(B组),IL-10基因修饰CD4+T细胞治疗组(C组),LacZ基因修饰的CD4+T细胞治疗组(D组),未经任何基因修饰的CD4+T细胞治疗组(E组),生理盐水治疗组(F组),应用卵白蛋白激发的哮喘模型。应用流式细胞仪分离小鼠外周血CD4+T细胞,基因转染采用重组腺病毒表达载体。应用肺组织标本冰冻切片免疫组化染色以确定肺气道NF-κB的表达。结果:IL-10基因转染的CD4+T细胞在第7天表达IL-10最高,并可以持续维持高水平表达40d左右。C组与D组和E组NF-κB表达经统计学处理差异均具有显著性(P<0.05)。结论:IL-10基因转染的CD4+T细胞使哮喘小鼠气道NF-κB表达下降,提示IL-10治疗哮喘可能通过抑制NF-κB传导有关。
Objective To investigate the effects of CD4^＋T cell modified by IL-10 on nuclear factor kappa expression of asthmatic airway in asthmatic mice and to probe into the influence mechanism of IL-10 on asthma. Method BALB mice were randomly divided into six groups：group A（control group） , group B（asthmatic model group）,group C（ cured by CD4^＋T cell modified by IL-10）,group D （cured by CD4^＋T cell modified by LacZ）,group E （cured by CD4^＋T cell without modified by any gene）,group F （cured by saline）. The asthmatic model was established in group B and C by egg white products,separating CD4^＋T cell with Flow cytometry,adopting recombined adenovirus vector to modify gene .The expression of NF-κB in the lung was analyzed by frozen section immunohistochemistry pigmention. Results The CD4^＋T cell modified by IL-10 has the most high level at the seventh day and maintain about 40 days. The difference of expression of NF-κB in the lung is significant between group C group B and group F（P〈0.05）. Conclusion The CD4^＋T cell modified by IL-10 make the low expression of NF-κB in the lung of asthmatic mice , and its mechamism maybe explained by fact that IL-10 inhibit the activation of NF-κB in the murine asthmatic model.
出处
《吉林医学》
CAS
2005年第8期826-827,829,共3页
Jilin Medical Journal
