摘要
目的:研究星状神经节阻滞(SGB)后甲醛诱导兔伤害的行为反应、炎性反应和间脑Fos表达,旨在探讨SGB对炎性疼痛的影响及其机制。方法:在兔星状神经节附近置入导管1周后,选择恢复健康兔24只,随机分为三组:即假手术组、SGB组和对照组,每组8只。SCB组和对照组在右前肢足底皮下注射3%甲醛0.5ml致痛,假手术组于相同部位注射等量等渗盐水。致痛前10min,SCB组经导管给予0.25%布比卡因0.5ml,假手术和对照组给等量等渗盐水。致痛2h后处死动物,并经升主动脉灌注甲醛溶液固定,取致痛局部组织和左右侧间脑。采用加权法对伤害行为反应评分,组织病理学方法观察局部炎性反应,免疫组化法检测间脑Fos表达。结果:SGB后甲醛诱导的Ⅱ期伤害行为反应和局部炎性反应明显缓解;SGB组下丘脑Fos样阳性神经元数明显少于对照组(P<0.01),而SGB组与对照组丘脑Fos样阳性神经元数的差异无显著性意义(P>0.05)。结论:SGB可减轻甲醛诱导的Ⅱ期伤害行为反应和局部炎性反应,降低下丘脑Fos表达,这可能与其治疗炎性痛的机制有关。
Objective： To investigate formalin-induced inflammatory nociceptive response and Fos expression of interbrain following stellate ganglion block （SGB） in rabbits. Methods ： Catheters were inserted closely to right stellate ganglia in rabbits. One week later,twenty-four rabbits were randomly divided into three groups of 8 each ： sham group, SGB group and control group, receiving O. 5 ml of normal saline, 3% formalin and 3% formalin stimulation by intraplantar injection into the right front paw, respectively. ten rains before stimulation,0.5 ml of 0.25% bupivacaine was administered via the catheter in SGB group,while 0.5 ml of normal saline in sham group and control group. Nociceptive response was observed for 60 rain using weighted pain score. Local inflammatory response was measured by histopathology, the expression of Fos of interbrain was measured with immunohistochemistry 2 hours after stimulation. Results： Formalin-induced nociceptive behavior in phase 2 and local inflammatory response were relieved following SGB; the average numbers of Fos-positive neurons of hypothalamus in SGB group was lower than that in control group（ P 〈0. 01 ）, while there was no significant difference in the average numbers of Fos-positive neurons of thalamus between both groups （ P 〉 . 05 ）. Conclusion ： SGB suppresses formalin-induced Fos expression of hypothalamus.
出处
《医学研究生学报》
CAS
2005年第8期692-695,i0015,共5页
Journal of Medical Postgraduates
基金
湖北省科技攻关计划重点项目基金资助(批准号:2003AA301C116)
