摘要
目的:观察高压氧(HBO)对骨髓移植时急性移植物抗宿主病(aGVHD)小鼠脾组织中细胞因子表达的影响,探讨HBO抗aGVHD的作用机制。方法:应用直线加速器排空C57BL/6小鼠骨髓后,注入BALB/C小鼠骨髓与脾淋巴细胞的混合液,复制aGVHD模型。应用RT-PCR技术检测经HBO、环孢素A(CsA)、氨甲喋呤(MTX)处理后,小鼠脾组织中白细胞介素2(IL-2)、肿瘤坏死因子α(TNF-α)、白细胞介素4(IL-4)及白细胞介素10(IL-10)mRNA水平的变化。结果:HBO组小鼠11d的生存率明显高于异基因移植组及CsA+MTX组;HBO组小鼠脾组织中TNF-α和IL-2mRNA相对含量显著低于异基因移植组(P<0.05)、显著高于CsA+MTX组;HBO组小鼠脾组织中IL-4和IL-10mRNA相对含量显著高于异基因移植组及CsA+MTX组(P<0.05)。结论:HBO可能通过调节促炎/抑炎细胞因子的分泌而发挥其明显的抗aGVHD作用。
Objective： To observe the effect of hyperbaric oxygen （ HBO ） on the expression of interleukin- 2 （IL-2）, tumor necrosis factor-α（ TNF-α）, interleukin-4（IL-4）, and interleukin-10（IL-10） in the spleen cells of mice with acute graft-versus-host diseasc（aGVHD） , and to study the inhibiting effect of HBO on aGVHD. Methods： The mice model of aGVHD was made by injecting the mixture of bone marrow and spleen cells from donor BALB/C mice into host C57 BL/6 mice after the host mice were exposed to total body radiation （TBI） by using linear accelerator. Then the levels of IL-2, TNF-α, IL-4, and IL-10 in the spleen cells were determined by RT-PCR after the mice were treated with HBO, cyclosporine A （CsA）, and methotrexate （MTX）. Results： The survival rate in HBO group was much higher than that in aUogenetic bone marrow transplantation （allo-BMT） group and CsA ＋ MTX group; The levels of IL-2 and TNF-α mRNA in HBO group were much lower than those in allo-BMT group but were much higher than those in CsA ＋ MTX group; The levels of IL-2, TNF-α, IL-4, and IL-10 in HBO group were much higher than those in CsA ＋ MTX group. Conclusion： HBO might inhibit aGVHD by regulating the secretion of cytokines。
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2005年第4期289-291,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(39570778)
关键词
高压氧
急性移植物抗宿主病
细胞因子
hyperbaric oxygen
acute graft-versus-host disease
cytokine
